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2013-03-13Zeitschriftenartikel DOI: 10.1371/journal.pone.0058021
Identification of a Novel Human Polyomavirus in Organs of the Gastrointestinal Tract
dc.contributor.authorKorup, Sarah
dc.contributor.authorRietscher, Janita
dc.contributor.authorCalvignac-Spencer, Sébastien
dc.contributor.authorTrusch, Franziska
dc.contributor.authorHofmann, Jörg
dc.contributor.authorMoens, Ugo
dc.contributor.authorSauer, Igor
dc.contributor.authorVoigt, Sebastian
dc.contributor.authorSchmuck, Rosa
dc.contributor.authorEhlers, Bernhard
dc.date.accessioned2018-05-07T16:20:05Z
dc.date.available2018-05-07T16:20:05Z
dc.date.created2013-03-27
dc.date.issued2013-03-13none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reoXQLMDdIxWI/PDF/21IJqtwk4uW9M.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1439
dc.description.abstractPolyomaviruses are small, non-enveloped viruses with a circular double-stranded DNA genome. Using a generic polyomavirus PCR targeting the VP1 major structural protein gene, a novel polyomavirus was initially identified in resected human liver tissue and provisionally named Human Polyomavirus 12 (HPyV12). Its 5033 bp genome is predicted to encode large and small T antigens and the 3 structural proteins VP1, VP2 and VP3. Phylogenetic analyses did not reveal a close relationship to any known human or animal polyomavirus. Investigation of organs, body fluids and excretions of diseased individuals and healthy subjects with both HPyV12-specific nested PCR and quantitative real-time PCR revealed additional virus-positive samples of resected liver, cecum and rectum tissues and a positive fecal sample. A capsomer-based IgG ELISA was established using the major capsid protein VP1 of HPyV12. Seroprevalences of 23% and 17%, respectively, were determined in sera from healthy adults and adolescents and a pediatric group of children. These data indicate that the virus naturally infects humans and that primary infection may already occur in childhood.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectHumanseng
dc.subjectMolecular Sequence Dataeng
dc.subjectAdolescenteng
dc.subjectAdulteng
dc.subjectAgedeng
dc.subjectMiddle Agedeng
dc.subjectYoung Adulteng
dc.subjectPrevalenceeng
dc.subjectPhylogenyeng
dc.subjectChild Preschooleng
dc.subjectChildeng
dc.subjectGastrointestinal Tract/virologyeng
dc.subjectGene Ordereng
dc.subjectGenome Viraleng
dc.subjectLiver/virologyeng
dc.subjectOpen Reading Frameseng
dc.subjectPolyomavirus/classificationeng
dc.subjectPolyomavirus/geneticseng
dc.subjectPolyomavirus/immunologyeng
dc.subjectPolyomavirus Infections/epidemiologyeng
dc.subjectPolyomavirus Infections/virologyeng
dc.subjectSeroepidemiologic Studieseng
dc.subjectTumor Virus Infections/epidemiologyeng
dc.subjectTumor Virus Infections/virologyeng
dc.subjectViral Proteins/geneticseng
dc.subject.ddc610 Medizin
dc.titleIdentification of a Novel Human Polyomavirus in Organs of the Gastrointestinal Tract
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10029836
dc.identifier.doi10.1371/journal.pone.0058021
dc.identifier.doihttp://dx.doi.org/10.25646/1364
local.edoc.container-titlePLoS One
local.edoc.container-textKorup S, Rietscher J, Calvignac-Spencer S, Trusch F, Hofmann J, et al. (2013) Identification of a Novel Human Polyomavirus in Organs of the Gastrointestinal Tract. PLoS ONE 8(3): e58021.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0058021
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume8
local.edoc.container-issue3
local.edoc.container-year2013

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