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2015-11-24Zeitschriftenartikel DOI: 10.1556/004.2015.047
Random sampling of the Central European bat fauna reveals the existence of numerous hitherto unknown adenoviruses
dc.contributor.authorVidovszky, Márton Z.
dc.contributor.authorKohl, Claudia
dc.contributor.authorBoldogh, Sándor
dc.contributor.authorGörföl, Tamás
dc.contributor.authorWibbelt, Gudrun
dc.contributor.authorKurth, Andreas
dc.contributor.authorHarrach, Balázs
dc.date.accessioned2018-05-07T18:45:37Z
dc.date.available2018-05-07T18:45:37Z
dc.date.created2015-12-21
dc.date.issued2015-11-24none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reAnvslQuf2zg/PDF/209LCXnFkuQ6.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2228
dc.description.abstractFrom over 1250 extant species of the order Chiroptera, 25 and 28 are known to occur in Germany and Hungary, respectively. Close to 350 samples originating from 28 bat species (17 from Germany, 27 from Hungary) were screened for the presence of adenoviruses (AdVs) using a nested PCR that targets the DNA polymerase gene of AdVs. An additional PCR was designed and applied to amplify a fragment from the gene encoding the IVa2 protein of mastadenoviruses. All German samples originated from organs of bats found moribund or dead. The Hungarian samples were excrements collected from colonies of known bat species, throat or rectal swab samples, taken from live individuals that had been captured for faunistic surveys and migration studies, as well as internal organs of dead specimens. Overall, 51 samples (14.73%) were found positive. We detected 28 seemingly novel and six previously described bat AdVs by sequencing the PCR products. The positivity rate was the highest among the guano samples of bat colonies. In phylogeny reconstructions, the AdVs detected in bats clustered roughly, but not perfectly, according to the hosts’ families (Vespertilionidae, Rhinolophidae, Hipposideridae, Phyllostomidae and Pteropodidae). In a few cases, identical sequences were derived from animals of closely related species. On the other hand, some bat species proved to harbour more than one type of AdV. The high prevalence of infection and the large number of chiropteran species worldwide make us hypothesise that hundreds of different yet unknown AdV types might circulate in bats.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectphylogenyeng
dc.subjectMolecular virologyeng
dc.subjectbat viruseseng
dc.subjectadenoviruseng
dc.subject.ddc610 Medizin
dc.titleRandom sampling of the Central European bat fauna reveals the existence of numerous hitherto unknown adenoviruses
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10042504
dc.identifier.doi10.1556/004.2015.047
dc.identifier.doihttp://dx.doi.org/10.25646/2153
local.edoc.container-titleActa Veterinaria Hungarica
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.akademiai.com/doi/abs/10.1556/004.2015.047
local.edoc.container-publisher-nameAkadémiai Kiadó
local.edoc.container-volume63
local.edoc.container-issue4
local.edoc.container-year2015

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