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2017-01-18Zeitschriftenartikel DOI: 10.1371/ journal.pntd.0005223
MLST-Based Population Genetic Analysis in a Global Context Reveals Clonality amongst Cryptococcus neoformans var. grubii VNI Isolates from HIV Patients in Southeastern Brazil
dc.contributor.authorFerreira-Paim, Kennio
dc.contributor.authorAndrade-Silva, Leonardo
dc.contributor.authorFonseca, Fernanda M.
dc.contributor.authorFerreira, Thatiana B.
dc.contributor.authorMora, Delio J.
dc.contributor.authorAndrade-Silva, Juliana
dc.contributor.authorKhan, Aziza
dc.contributor.authorDao, Aiken
dc.contributor.authorReis, Eduardo C.
dc.contributor.authorAlmeida, Margarete T. G.
dc.contributor.authorMaltos, Andre
dc.contributor.authorJunior, Virmondes R.
dc.contributor.authorTrilles, Luciana
dc.contributor.authorRickerts, Volker
dc.contributor.authorChindamporn, Ariya
dc.contributor.authorSykes, Jane E.
dc.contributor.authorCogliati, Massimo
dc.contributor.authorNielsen, Kirsten
dc.contributor.authorBoekhout, Teun
dc.contributor.authorFisher, Matthew
dc.contributor.authorKwon-Chung, June
dc.contributor.authorEngelthaler, David M.
dc.contributor.authorLazéra, Marcia
dc.contributor.authorMeyer, Wieland
dc.contributor.authorSilva-Vergara, Mario L.
dc.date.accessioned2018-05-07T19:44:51Z
dc.date.available2018-05-07T19:44:51Z
dc.date.created2017-02-14
dc.date.issued2017-01-18none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reGbC5Fvy8WcQ/PDF/27AwVa5GDJ5I.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2551
dc.description.abstractCryptococcosis is an important fungal infection in immunocompromised individuals, especially those infected with HIV. In Brazil, despite the free availability of antiretroviral therapy (ART) in the public health system, the mortality rate due to Cryptococcus neoformans meningitis is still high. To obtain a more detailed picture of the population genetic structure of this species in southeast Brazil, we studied 108 clinical isolates from 101 patients and 35 environmental isolates. Among the patients, 59% had a fatal outcome mainly in HIV-positive male patients. All the isolates were found to be C. neoformans var. grubii major molecular type VNI and mating type locus alpha. Twelve were identified as diploid by flow cytometry, being homozygous (AαAα) for the mating type and by PCR screening of the STE20, GPA1, and PAK1 genes. Using the ISHAM consensus multilocus sequence typing (MLST) scheme, 13 sequence types (ST) were identified, with one being newly described. ST93 was identified from 81 (75%) of the clinical isolates, while ST77 and ST93 were identified from 19 (54%) and 10 (29%) environmental isolates, respectively. The southeastern Brazilian isolates had an overwhelming clonal population structure. When compared with populations from different continents based on data extracted from the ISHAM-MLST database (mlst.mycologylab.org) they showed less genetic variability. Two main clusters within C. neoformans var. grubii VNI were identified that diverged from VNB around 0.58 to 4.8 million years ago.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subject.ddc610 Medizin
dc.titleMLST-Based Population Genetic Analysis in a Global Context Reveals Clonality amongst Cryptococcus neoformans var. grubii VNI Isolates from HIV Patients in Southeastern Brazil
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10051231
dc.identifier.doi10.1371/ journal.pntd.0005223
dc.identifier.doihttp://dx.doi.org/10.25646/2476
local.edoc.container-titlePLoS Neglected Tropical Diseases
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005223
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume11
local.edoc.container-issue1
local.edoc.container-year2017

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