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2018-03-22Zeitschriftenartikel DOI: 10.3389/fcimb.2018.00089
Population Genomics of Francisella tularensis subsp. holarctica and its Implication on the Eco-Epidemiology of Tularemia in Switzerland
dc.contributor.authorWittwer, Matthias
dc.contributor.authorAltpeter, Ekkehard
dc.contributor.authorPilo, Paola
dc.contributor.authorGygli, Sebastian M.
dc.contributor.authorBeuret, Christian
dc.contributor.authorFoucault, Frederic
dc.contributor.authorAckermann-Gäumann, Rahel
dc.contributor.authorKarrer, Urs
dc.contributor.authorJacob, Daniela
dc.contributor.authorGrunow, Roland
dc.contributor.authorSchürch, Nadia
dc.date.accessioned2018-05-07T21:19:08Z
dc.date.available2018-05-07T21:19:08Z
dc.date.created2018-04-20
dc.date.issued2018-03-22none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reGjeDW0CHRvk/PDF/26hGQfnHrG07o.pdf
dc.identifier.urihttp://edoc.rki.de/176904/3058
dc.description.abstractWhole genome sequencing (WGS) methods provide new possibilities in the field of molecular epidemiology. This is particularly true for monomorphic organisms where the discriminatory power of traditional methods (e.g., restriction enzyme length polymorphism typing, multi locus sequence typing etc.) is inadequate to elucidate complex disease transmission patterns, as well as resolving the phylogeny at high resolution on a micro-geographic scale. In this study, we present insights into the population structure of Francisella tularensis subsp. holarctica, the causative agent of tularemia in Switzerland. A total of 59 Fth isolates were obtained from castor bean ticks (Ixodes ricinus), animals and humans and a high resolution phylogeny was inferred using WGS methods. The majority of the Fth population in Switzerland belongs to the west European B.11 clade and shows an extraordinary genetic diversity underlining the old evolutionary history of the pathogen in the alpine region. Moreover, a new B.11 subclade was identified which was not described so far. The combined analysis of the epidemiological data of human tularemia cases with the whole genome sequences of the 59 isolates provide evidence that ticks play a pivotal role in transmitting Fth to humans and other vertebrates in Switzerland. This is further underlined by the correlation of disease risk estimates with climatic and ecological factors influencing the survival of ticks.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectphylogenomicseng
dc.subjecttularemiaeng
dc.subjectwhole genome sequencing (WGS)eng
dc.subjecttickseng
dc.subjectFrancisella tularensis subsp. holarcticaeng
dc.subjectecologyeng
dc.subjectepidemiology of infectious diseaseseng
dc.subjectcanSNPseng
dc.subject.ddc610 Medizin
dc.titlePopulation Genomics of Francisella tularensis subsp. holarctica and its Implication on the Eco-Epidemiology of Tularemia in Switzerland
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10058650
dc.identifier.doi10.3389/fcimb.2018.00089
dc.identifier.doihttp://dx.doi.org/10.25646/2983
local.edoc.container-titleFrontiers in Cellular and Infection Microbiology
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.frontiersin.org/articles/10.3389/fcimb.2018.00089/full
local.edoc.container-publisher-nameFrontiers Media
local.edoc.container-volume8
local.edoc.container-issue89
local.edoc.container-year2018

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