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Browsing by Author "Klotz, Christian"
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2017-11-09ZeitschriftenartikelA European network for food-borne parasites (Euro-FBP): meeting report on ‘Analytical methods for food-borne parasites in human and veterinary diagnostics and in food matrices’ Klotz, Christian; Šoba, Barbara; Skvarč, Miha; Gabriël, Sarah; Robertson, Lucy J.Food-borne parasites (FBPs) are a neglected topic in food safety, partly due to a lack of awareness of their importance for public health, especially as symptoms tend not to develop immediately after exposure. In addition, ...
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2021-11-23ZeitschriftenartikelDissection of Barrier Dysfunction in Organoid-Derived Human Intestinal Epithelia Induced by Giardia duodenalis Holthaus, David; Kraft, Martin R.; Krug, Susanne M.; Wolf, Silver; Müller, Antonia; Delgado-Betancourt, Estefanía; Schorr, Madeleine; Holland, Gudrun; Knauf, Felix; Schulzke, Joerg-Dieter; Aebischer, Toni; Klotz, ChristianBACKGROUND & AIMS: The protozoa Giardia duodenalis is a major cause of gastrointestinal illness worldwide, but un- derlying pathophysiological mechanisms remain obscure, partly due to the absence of adequate cellular ...
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2014-07-11ZeitschriftenartikelEpidemiology of Giardia duodenalis infection in ruminant livestock and children in the Ismailia province of Egypt: insights by genetic characterization Helmy, Yosra A.; Klotz, Christian; Wilking, Hendrik; Krücken, Jürgen; Nöckler, Karsten; Samson-Himmelstjerna, Georg von; Zessin, Karl-H.; Aebischer, ToniBackground: Giardia duodenalis is a common flagellated protozoan parasite that infects the small intestine of a wide range of vertebrate hosts. This study aimed to determine whether tracing of G. duodenalis isolates by ...
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2022-12-26ZeitschriftenartikelExtensive testing of a multi-locus sequence typing scheme for Giardia duodenalis assemblage A confirms its good discriminatory power Klotz, Christian; Sannella, Anna Rosa; Weisz, Filip; Chaudhry, Umer; Sroka, Jacek; Tůmová, Pavla; Nohýnková, Eva; Ignatius, Ralf; Aebischer, Toni; Betson, Martha; Troell, Karin; Cacciò, SimoneBackground The flagellated parasite Giardia duodenalis is a major and global cause of diarrhoeal disease. Eight genetically very distinct groups, known as assemblages A to H, have been recognized in the G. duodenalis ...
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2019-03-05ZeitschriftenartikelFrom Entry to Early Dissemination—Toxoplasma gondii's Initial Encounter With Its Host Delgado Betancourt, Estefania; Hamid, Benjamin; Fabian, Benedikt T.; Klotz, Christian; Hartmann, Susanne; Seeber, FrankToxoplasma gondii is a zoonotic intracellular parasite, able to infect any warm-blooded animal via ingestion of infective stages, either contained in tissue cysts or oocysts released into the environment. While immune ...
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2022-12-21ZeitschriftenartikelGenetic diversity in the metronidazole metabolism genes nitroreductases and pyruvate ferredoxin oxidoreductases in susceptible and refractory clinical samples of Giardia lamblia Saghuag, Christina S.; Gamlem, Astrid L.; Hauge, Kirsti B.; Vahokoski, Juha; Klotz, Christian; Langeland, Nina; Hanevik, Kurt; Aebischer, ToniThe effectiveness of metronidazole against the tetraploid intestinal parasite Giardia lamblia is dependent on its activation/inactivation within the cytoplasm. There are several activating enzymes, including pyruvate ...
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2020-12-18ZeitschriftenartikelGenetic Diversity of the Flavohemoprotein Gene of Giardia lamblia: Evidence for High Allelic Heterozygosity and Copy Number Variation Saghaug, C. S.; Klotz, Christian; Kallio, J. P.; Aebischer, Toni; Langeland, N.; Hanevik, K.Purpose: The flavohemoprotein (gFlHb) in Giardia plays an important role in managing nitrosative and oxidative stress, and potentially also in virulence and nitroimidazole drug tolerance. The aim of this study was to analyze ...
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2019-05-10ZeitschriftenartikelGenetic variation in metronidazole metabolism and oxidative stress pathways in clinical Giardia lamblia assemblage A and B isolates Saghaug, Christina S.; Klotz, Christian; Kallio, Juha P.; Brattbakk, Hans-Richard; Stokowy, Tomasz; Aebischer, Toni; Kursula, Inari; Langeland, Nina; Hanevik, KurtPurpose: Treatment-refractory Giardia cases have increased rapidly within the last decade. No markers of resistance nor a standardized susceptibility test have been established yet, but several enzymes and their pathways ...
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2013-04-15ZeitschriftenartikelGiardia duodenalis arginine deiminase modulates the phenotype and cytokine secretion of human dendritic cells by depletion of arginine and formation of ammonia Banik, Stefanie; Viveros, Pablo Renner; Seeber, Frank; Klotz, Christian; Ignatius, Ralf; Aebischer, ToniDepletion of arginine is a recognized strategy that pathogens use to evade immune effector mechanisms. Depletion depends on microbial enzymes such as arginases, which are considered virulence factors. The effect is mostly ...
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2017-09-26ZeitschriftenartikelGiardia's Epithelial Cell Interaction In Vitro: Mimicking Asymptomatic Infection? Kraft, Martin R.; Klotz, Christian; Bücker, Roland; Schulzke, Jörg-Dieter; Aebischer, ToniThe protozoan parasite Giardia duodenalis is responsible for more than 280 million cases of gastrointestinal complaints (“giardiasis”) every year, worldwide. Infections are acquired orally, mostly via uptake of cysts in ...
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2021-02-22ZeitschriftenartikelHarmonization of Protocols for Multi-Species Organoid Platforms to Study the Intestinal Biology of Toxoplasma gondii and Other Protozoan Infections Holthaus, David; Delgado-Betancourt, Estefanía; Aebischer, Toni; Seeber, Frank; Klotz, ChristianThe small intestinal epithelium is the primary route of infection for many protozoan parasites. Understanding the mechanisms of infection, however, has been hindered due to the lack of appropriate models that recapitulate ...
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2012-06-12ZeitschriftenartikelHigh Prevalence of Giardia duodenalis Assemblage B Infection and Association with Underweight in Rwandan Children Ignatius, Ralf; Gahutu, Jean Bosco; Klotz, Christian; Steininger, Christian; Shyirambere, Cyprien; Lyng, Michel; Musemakweri, Andre; Aebischer, Toni; Martus, Peter; Harms, Gundel; Mockenhaupt, Frank P.Background: Giardia duodenalis is highly endemic in East Africa but its effects on child health, particularly of submicroscopic infections, i.e., those below the threshold of microscopy, and of genetic subgroups ...
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2013-08-19ZeitschriftenartikelHigh Sensitivity of Giardia duodenalis to Tetrahydrolipstatin (Orlistat) In Vitro Hahn, Juliane; Seeber, Frank; Kolodziej, Herbert; Ignatius, Ralf; Laue, Michael; Aebischer, Toni; Klotz, ChristianGiardiasis, a gastrointestinal disease caused by Giardia duodenalis, is currently treated mainly with nitroimidazoles, primarily metronidazole (MTZ). Treatment failure rates of up to 20 percent reflect the compelling need ...
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2022-06-06ZeitschriftenartikelHuman duodenal organoid-derived monolayers serve as a suitable barrier model for duodenal tissue Weiß, Franziska; Holthaus, David; Kraft, Martin; Klotz, Christian; Schneemann, Martina; Schulzke, Jörg D.; Krug, Susanne M.Usually, duodenal barriers are investigated using intestinal cell lines like Caco-2, which in contrast to native tissue are limited in cell-type representation. Organoids can consist of all intestinal cell types and are ...
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2013-11-04ZeitschriftenartikelIncreased expression of CD25, CD83, and CD86, and secretion of IL-12, IL-23, and IL-10 by human dendritic cells incubated in the presence of Toll-like receptor 2 ligands and Giardia duodenalis Obendorf, Janine; Viveros, Pablo Renner; Fehlings, Michael; Klotz, Christian; Aebischer, Toni; Ignatius, RalfBackground: Effects of Giardia duodenalis on dendritic cell (DC) functions may contribute to the pathogenesis of chronic giardiasis. G. duodenalis lysate has been shown to inhibit the activation of murine DCs through ...
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2021-11-09ZeitschriftenartikelNF-κB determines Paneth versus goblet cell fate decision in the small intestine Brischetto, Cristina; Krieger, Karsten; Klotz, Christian; Krahn, Inge; Kunz, Séverine; Kolesnichenko, Marina; Mucka, Patrick; Heuberger, Julian; Scheidereit, Claus; Schmidt-Ullrich, RuthAlthough the role of the transcription factor NF-κB in intestinal inflammation and tumor formation has been investigated extensively, a physiological function of NF-κB in sustaining intestinal epithelial homeostasis beyond ...
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2018-03-27ZeitschriftenartikelOccurrence and distribution of Giardia species in wild rodents in Germany Helmy, Yosra A.; Spierling, Nastasja G.; Schmidt, Sabrina; Rosenfeld, Ulrike M.; Reil, Daniela; Imholt, Christian; Jacob, Jens; Ulrich, Rainer G.; Aebischer, Toni; Klotz, ChristianBackground: Giardiasis is an important gastrointestinal parasitic disease in humans and other mammals caused by the protozoan Giardia duodenalis. This species complex is represented by genetically distinct groups ...
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2020-05-13ZeitschriftenartikelParasite detection in food: Current status and future needs for validation Chalmers, Rachel M.; Robertson, Lucy J.; Dorny, Pierre; Jordan, Suzanne; Kärssin, Age; Katzer, Frank; La Carbona, Stéphanie; Lalle, Marco; Lassen, Brian; Mladineo, Ivona; Rozycki, Miroslaw; Bilska-Zajac, Ewa; Schares, Gereon; Mayer-Scholl, Anne; Trevisan, Chiara; Tysnes, Kristoffer; Vasilev, Sasa; Klotz, ChristianBackground Many parasites (protozoa and helminths) can be transmitted through food and lead to infections with high morbidity, as well as disease outbreaks. Although the importance of foodborne parasites (FBP) is recognised ...
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2021-07-18ZeitschriftenartikelReal-Time PCR for Molecular Detection of Zoonotic and Non-Zoonotic Giardia spp. in Wild Rodents Klotz, Christian; Radam, Elke; Rausch, Sebastian; Gosten-Heinrich, Petra; Aebischer, ToniGiardiasis in humans is a gastrointestinal disease transmitted by the potentially zoonotic Giardia duodenalis genotypes (assemblages) A and B. Small wild rodents such as mice and voles are discussed as potential reservoirs ...
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2018-02-12ZeitschriftenartikelRecombinant IFN-γ from the bank vole Myodes glareolus: a novel tool for research on rodent reservoirs of zoonotic pathogens Torelli, Francesca; Zander, Steffen; Ellerbrok, Heinz; Kochs, Georg; Ulrich, Rainer G.; Klotz, Christian; Seeber, FrankRodent species like Myodes glareolus and Microtus spp. are natural reservoirs for many zoonotic pathogens causing human diseases and are gaining increasing interest in the field of eco-immunology as candidate animal models. ...