Browsing by Author "Uddin, Steven"

Now showing items 1-4 of 4

2021-07-01Zeitschriftenartikel

AmpliCoV: Rapid Whole-Genome Sequencing Using Multiplex PCR Amplification and Real-Time Oxford Nanopore MinION Sequencing Enables Rapid Variant Identification of SARS-CoV-2

Brinkmann, Annika; Ulm, Sophie-Luisa; Uddin, Steven; Förster, Sophie; Seifert, Dominique; Oehme, Rainer; Corty, Merle; Schaade, Lars; Michel, Janine; Nitsche, Andreas

Since the emergence of the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) in December 2019, the scientific community has been sharing data on epidemiology, diagnostic methods, and whole-genomic sequences ...
2024-01-20Zeitschriftenartikel

Genome sequencing of the mpox virus 2022 outbreak with amplicon-based Oxford Nanopore MinION sequencing

Brinkmann, Annika; Pape, Katharina; Uddin, Steven; Woelk, Niklas; Förster, Sophie; Jessen, Heiko; Michel, Janine; Kohl, Claudia; Schaade, Lars; Nitsche, Andreas

We present an amplicon-based assay for MinION Nanopore sequencing of mpox virus (MPXV) genomes from clinical specimens, obtaining high-quality results with an average genome coverage of 99% for Ct values of up to 25, and ...
2022-03-09Zeitschriftenartikel

RespiCoV: Simultaneous identification of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and 46 respiratory tract viruses and bacteria by amplicon-based Oxford-Nanopore MinION sequencing

Brinkmann, Annika; Uddin, Steven; Ulm, Sophie-Luisa; Pape, Katharina; Förster, Sophie; Enan, Khalid; Nourlil, Jalal; Krause, Eva; Schaade, Lars; Michel, Janine; Nitsche, Andreas

Since December 2019 the world has been facing the outbreak of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Identification of infected patients and discrimination from other respiratory infections have ...
2021-01-29Zeitschriftenartikel

Utility of a Sequence-Independent, Single-Primer-Amplification (SISPA) and Nanopore Sequencing Approach for Detection and Characterization of Tick-Borne Viral Pathogens

Brinkmann, Annik; Uddin, Steven; Krause, Eva; Surtees, Rebecca; Dinçer, Ender; Kar, Sırrı; Hacıoğlu, Sabri; Özkul, Aykut; Ergünay, Koray; Nitsche, Andreas

Currently, next generation sequencing (NGS) is the mainly used approach for identification and monitorization of viruses with a potential public health threat in clinical and environmental samples. To facilitate detection ...