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Browsing by Author "Walter, Steffi"
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2018-02-15ZeitschriftenartikelHigh-Throughput Quantification of Bacterial-Cell Interactions Using Virtual Colony Counts Hoffmann, Stefanie; Walter, Steffi; Blume, Anne-Kathrin; Fuchs, Stephan; Schmidt, Christiane; Scholz, Annemarie; Gerlach, Roman G.The quantification of bacteria in cell culture infection models is of paramount importance for the characterization of host-pathogen interactions and pathogenicity factors involved. The standard to enumerate bacteria in ...
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2017-02-17ZeitschriftenartikelScarless deletion of up to seven methyl-accepting chemotaxis genes with an optimized method highlights key function of CheM in Salmonella Typhimurium Hoffmann, Stefanie; Schmidt, Christiane; Walter, Steffi; Bender, Jennifer K.; Gerlach, Roman G.Site-directed scarless mutagenesis is an essential tool of modern pathogenesis research. We describe an optimized two-step protocol for genome editing in Salmonella enterica serovar Typhimurium to enable multiple sequential ...