Auflistung nach Schlagwort "Polymerase Chain Reaction/methods"
Anzeige der Publikationen 1-20 von 24
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2003-06-10ZeitschriftenartikelApproaching virus safety in xenotransplantation: A search for unrecognized herpesviruses in pigs Abstract: The identification of porcine viruses so far unrecognized is required to minimize virus-related risks associated with xenotransplantation. We used a pan-herpes consensus polymerase chain reaction assay to search ...
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2011-01-27ZeitschriftenartikelCases of community-acquired meticillin-resistant Staphylococcus aureus in an asylum seekers centre in Germany, November 2010 In an asylum seeker centre in Schleswig-Holstein, a resident was diagnosed with furuncle caused by a Panton-Valentine leukocidine (PVL)-positive community- acquired meticillin-resistant Staphylococcus aureus (CA-MRSA). ...
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2008-12-03ZeitschriftenartikelDetection of Balamuthia mandrillaris DNA by real-time PCR targeting the RNase P gene Background: The free-living amoeba Balamuthia mandrillaris may cause fatal encephalitis both in immunocompromised and in – apparently – immunocompetent humans and other mammalian species. Rapid, specific, sensitive, and ...
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2006-07-01ZeitschriftenartikelDetection of Infectious Poxvirus Particles. To enable rapid and reliable detection of poxviruses in clinical and environmental specimens, a diagnostic approach was developed to detect
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2012-05-08ZeitschriftenartikelDetection of retroviral super-infection from non-invasive samples. While much attention has been focused on the molecular epidemiology of retroviruses in wild primate populations, the correlated question of the frequency and nature of super-infection events, i.e., the simultaneous infection ...
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2010-04-01ZeitschriftenartikelDiagnostic Approach for the Differentiation of the Pandemic Influenza A(H1N1)v Virus from Recent Human Influenza Viruses by Real-Time PCR Background: The current spread of pandemic influenza A(H1N1)v virus necessitates an intensified surveillance of influenza virus infections worldwide. So far, in many laboratories routine diagnostics were limited to ...
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2010-01-27ZeitschriftenartikelDifferentiation of influenza b virus lineages yamagata and victoria by real-time PCR Since the 1970s, influenza B viruses have diverged into two antigenically distinct virus lineages called the Yamagata and Victoria lineages. We present the first real-time PCR assay for virus lineage differentiation to ...
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2010-10-28ZeitschriftenartikelDifferentiation of Medically Important Euro-Asian Tick Species Ixodes ricinus, Ixodes persulcatus, Ixodes hexagonus, and Dermacentor reticulatus by Polymerase Chain Reaction Understanding epidemiology of the tick-borne pathogens requires the accurate identification of the vector ticks. Morphological analysis of ticks is difficult and often leads to misidentification. Molecular techniques offer ...
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2007-09-06ZeitschriftenartikelDiscovery of herpesviruses in multi-infected primates using locked nucleic acids (LNA) and a bigenic PCR approach Targeting the highly conserved herpes DNA polymerase (DPOL) gene with PCR using panherpes degenerate primers is a powerful tool to universally detect unknown herpesviruses. However, vertebrate hosts are often infected with ...
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2012-03-13ZeitschriftenartikelEnhanced fungal DNA-extraction from formalin-fixed, paraffin-embedded tissue specimens by application of thermal energy Determining the etiology of invasive fungal infections (IFI) is critical for patient management as fungi vary in their susceptibility to antifungals. However, the etiology remains obscure in many cases due to negative ...
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2009-11-26ZeitschriftenartikelHigh genotypic diversity and a novel variant of human cytomegalovirus revealed by combined UL33/UL55 genotyping with broad-range PCR The known strains of human cytomegalovirus (HCMV) represent genotypic variants of a single species, and HCMV genotypic variability has been studied in order to reveal correlations between different disease patterns and the ...
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2012-06-12ZeitschriftenartikelHigh Prevalence of Giardia duodenalis Assemblage B Infection and Association with Underweight in Rwandan Children Background: Giardia duodenalis is highly endemic in East Africa but its effects on child health, particularly of submicroscopic infections, i.e., those below the threshold of microscopy, and of genetic subgroups ...
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2011-02-10ZeitschriftenartikelHighly sensitive detection of the group A Rotavirus using Apolipoprotein H-coated ELISA plates compared to quantitative real-time PCR Background: The principle of a capture ELISA is binding of specific capture antibodies (polyclonal or monoclonal) to the surface of a suitable 96 well plate. These immobilized antibodies are capable of specifically binding ...
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2006-01-19ZeitschriftenartikelHighly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii Background: Coxiella burnetii, the bacterium causing Q fever, is an obligate intracellular biosafety level 3 agent. Detection and quantification of these bacteria with conventional methods is time consuming and dangerous. ...
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2009-12-21ZeitschriftenartikelIdentification of a novel betaherpesvirus in Mus musculus Rodent betaherpesviruses vary considerably in genomic content, and these variations can result in a distinct pathogenicity. Therefore, the identification of unknown betaherpesviruses in house mice (Mus musculus), the ...
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2012-08-14ZeitschriftenartikelIsolation and characterization of three Mammalian orthoreoviruses from European bats. In recent years novel human respiratory disease agents have been described in South East Asia and Australia. The causative pathogens were classified as pteropine orthoreoviruses with strong phylogenetic relationship to ...
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2009-10-16ZeitschriftenartikelLack of evidence for xenotropic murine leukemia virus-related virus(XMRV) in German prostate cancer patients Background: A novel gammaretrovirus named xenotropic murine leukemia virus-related virus (XMRV) has been recently identified and found to have a prevalence of 40% in prostate tumor samples from American patients carrying ...
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2009-11-18ZeitschriftenartikelLymphocryptovirus phylogeny and the origins of Epstein-Barr virus. Specimens from wild and captive primates were collected, and searched for new members of the genus Lymphocryptovirus (subfamily Gammaherpesvirinae) utilising PCR for the DNA polymerase gene. Twenty-one new viruses were ...
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2010-12-22ZeitschriftenartikelNo Evidence for XMRV in German CFS and MS Patients with Fatigue Despite the Ability of the Virus to Infect Human Blood Cells In Vitro Background: Xenotropic murine leukemia virus-related virus (XMRV), a novel human retrovirus originally identified in prostate cancer tissues, has recently been associated with chronic fatigue syndrome (CFS), a disabling ...
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2012-06-21ZeitschriftenartikelNovel paramyxoviruses in free-ranging European bats. The zoonotic potential of paramyxoviruses is particularly demonstrated by their broad host range like the highly pathogenic Hendra and Nipah viruses originating from bats. But while so far all bat-borne paramyxoviruses ...