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2023-10-05Zeitschriftenartikel
External quality assessment of orthohantavirus and lymphocytic choriomeningitis virus molecular detection and serology in Europe, 2021
dc.contributor.authorErdin, Mert
dc.contributor.authorStanoeva, Kamelia R.
dc.contributor.authorMögling, Ramona
dc.contributor.authorKorva, Miša
dc.contributor.authorKnap, Nataša
dc.contributor.authorResman Rus, Katarina
dc.contributor.authorDomingo, Cristina
dc.contributor.authorReimerink, Johan H. J.
dc.contributor.authorde Vries, Ankje
dc.contributor.authorAlburkat, Hussein
dc.contributor.authorUtriainen, Mira
dc.contributor.authorGossner, Céline M.
dc.contributor.authorSironen, Tarja
dc.contributor.authorAvšič-Županc, Tatjana
dc.contributor.authorReusken, Chantal B. E. M.
dc.contributor.authorVapalahti, Olli
dc.date.accessioned2023-11-13T14:47:49Z
dc.date.available2023-11-13T14:47:49Z
dc.date.issued2023-10-05none
dc.identifier.other10.2807/1560-7917.ES.2023.28.40.2300054
dc.identifier.urihttp://edoc.rki.de/176904/11340
dc.description.abstractBackground Rodent-borne viruses such as orthohantaviruses and arenaviruses cause considerable disease burden with regional and temporal differences in incidence and clinical awareness. Therefore, it is important to regularly evaluate laboratory diagnostic capabilities, e.g. by external quality assessments (EQA). Aim We wished to evaluate the performance and diagnostic capability of European expert laboratories to detect orthohantaviruses and lymphocytic choriomeningitis virus (LCMV) and human antibody response towards orthohantaviruses. Methods We conducted an EQA in 2021; molecular panels consisted of 12 samples, including different orthohantaviruses (Seoul, Dobrava-Belgrade (DOBV), Puumala (PUUV) and Hantaan orthohantavirus), LCMV and negative controls. Serological panels consisted of six human serum samples reactive to PUUV, DOBV or negative to orthohantaviruses. The EQA was sent to 25 laboratories in 20 countries. Results The accuracy of molecular detection of orthohantaviruses varied (50‒67%, average 62%) among 16 participating laboratories, while LCMV samples were successfully detected in all 11 participating laboratories (91-100%, average 96%). The accuracy of serological diagnosis of acute and past orthohantavirus infections was on average 95% among 20 participating laboratories and 82% in 19 laboratories, respectively. A variety of methods was used, with predominance of in-house assays for molecular tests, and commercial assays for serological ones. Conclusion Serology, the most common tool to diagnose acute orthohantavirus infections, had a high accuracy in this EQA. The molecular detection of orthohantaviruses needs improvement while LCMV detection (performed in fewer laboratories) had 95% accuracy. Further EQAs are recommended to be performed periodically to monitor improvements and challenges in the diagnostics of rodent–borne diseases.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectEuropeeng
dc.subjectexternal quality assessmenteng
dc.subjecthantaviruseseng
dc.subjectlymphocytic choriomeningitis viruseng
dc.subjectmolecular diagnosticeng
dc.subjectserologyeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleExternal quality assessment of orthohantavirus and lymphocytic choriomeningitis virus molecular detection and serology in Europe, 2021none
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11340-1
dc.type.versionpublishedVersionnone
local.edoc.container-titleEurosurveilancenone
local.edoc.container-issn1560-7917none
local.edoc.pages10none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.eurosurveillance.org/content/eurosurveillancenone
local.edoc.container-publisher-nameEuropean Centre for Disease Prevention and Controlnone
local.edoc.container-reportyear2023none
dc.description.versionPeer Reviewednone

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