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2023-06-01Zeitschriftenartikel
Direct comparison of clinical diagnostic sensitivity of saliva from buccal swabs versus combined oro-/nasopharyngeal swabs in the detection of SARS-CoV-2 B.1.1.529 Omicron
dc.contributor.authorPuyskens, Andreas
dc.contributor.authorMichel, Janine
dc.contributor.authorStoliaroff-Pepin, Anna
dc.contributor.authorBayram, Fatimanur
dc.contributor.authorSesver, Akin
dc.contributor.authorWichmann, Ole
dc.contributor.authorHarder, Thomas
dc.contributor.authorSchaade, Lars
dc.contributor.authorNitsche, Andreas
dc.contributor.authorPeine, Caroline
dc.date.accessioned2023-11-16T13:40:13Z
dc.date.available2023-11-16T13:40:13Z
dc.date.issued2023-06-01none
dc.identifier.other10.1016/j.jcv.2023.105496
dc.identifier.urihttp://edoc.rki.de/176904/11360
dc.description.abstractBackground/Purpose: While current guidelines recommend the use of respiratory tract specimens for the direct detection of SARS-CoV-2 infection, saliva has recently been suggested as preferred sample type for the sensitive detection of SARS-CoV-2 B.1.1.529 (Omicron). By comparing saliva collected using buccal swabs and oro-/ nasopharyngeal swabs from patients hospitalized due to COVID-19, we aimed at identifying potential differences in virus detection sensitivity between these sample types. Methods: We compare the clinical diagnostic sensitivity of paired buccal swabs and combined oro-/nasopha- ryngeal swabs from hospitalized, symptomatic COVID-19 patients collected at median six days after symptom onset by real-time polymerase chain reaction (PCR) and antigen test. Results: Of the tested SARS-CoV-2 positive sample pairs, 55.8% were identified as SARS-CoV-2 Omicron BA.1 and 44.2% as Omicron BA.2. Real-time PCR from buccal swabs generated significantly higher quantification cycle (Cq) values compared to those from matched combined oro-/nasopharyngeal swabs and resulted in an increased number of false-negative PCR results. Reduced diagnostic sensitivity of buccal swabs by real-time PCR was observed already at day one after symptom onset. Similarly, antigen test detection rates were reduced in buccal swabs compared to combined oro-/nasopharyngeal swabs. Conclusion: Our results suggest reduced clinical diagnostic sensitivity of saliva collected using buccal swabs when compared to combined oro-/nasopharyngeal swabs in the detection of SARS-CoV-2 Omicron in symptomatic individualseng
dc.description.abstractGraphische Zusammenfassung, als Datei angehängt.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectSARS-CoV-2eng
dc.subjectB.1.1.529(Omicron)eng
dc.subjectclinical diagnostic sensitivityeng
dc.subjectsalivaeng
dc.subjectbuccal swab samplingeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleDirect comparison of clinical diagnostic sensitivity of saliva from buccal swabs versus combined oro-/nasopharyngeal swabs in the detection of SARS-CoV-2 B.1.1.529 Omicronnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11360-1
dc.type.versionupdatedVersionnone
local.edoc.container-titleJournal of clinical Virologynone
local.edoc.container-issn1873-5967none
local.edoc.pages5none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.sciencedirect.com/journal/journal-of-clinical-virologynone
local.edoc.container-publisher-nameElseviernone
local.edoc.container-volume165none
local.edoc.container-issueAugut 2023none
local.edoc.container-reportyear2023none
dc.description.versionPeer Reviewednone

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