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2023-01-24Zeitschriftenartikel
Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patients
dc.contributor.authorMartin-Souto, Leire
dc.contributor.authorAntoran, Aitziber
dc.contributor.authorAreitio, Maialen
dc.contributor.authorAparicio-Fernandez, Leire
dc.contributor.authorMartín-Gómez, María Teresa
dc.contributor.authorFernandez, Roberto
dc.contributor.authorAstigarraga, Egoitz
dc.contributor.authorBarreda-Gómez, Gabriel
dc.contributor.authorSchwarz, Carsten
dc.contributor.authorRickerts, Volker
dc.contributor.authorHernando, Fernando L.
dc.contributor.authorRementeria, Aitor
dc.contributor.authorBuldain, Idoia
dc.contributor.authorRamirez-Garcia, Andoni
dc.date.accessioned2023-11-27T15:53:24Z
dc.date.available2023-11-27T15:53:24Z
dc.date.issued2023-01-24none
dc.identifier.other10.3390/jof9020158
dc.identifier.urihttp://edoc.rki.de/176904/11388
dc.description.abstractThe detection of Scedosporium/Lomentospora is still based on non-standardized low-sensitivity culture procedures. This fact is particularly worrying in patients with cystic fibrosis (CF), where these fungi are the second most common filamentous fungi isolated, because a poor and delayed diagnosis can worsen the prognosis of the disease. To contribute to the discovery of new diagnostic strategies, a rapid serological dot immunobinding assay (DIA) that allows the detection of serum IgG against Scedosporium/Lomentospora in less than 15 min was developed. A crude protein extract from the conidia and hyphae of Scedosporium boydii was employed as a fungal antigen. The DIA was evaluated using 303 CF serum samples (162 patients) grouped according to the detection of Scedosporium/Lomentospora in the respiratory sample by culture, obtaining a sensitivity and specificity of 90.48% and 79.30%, respectively; positive and negative predictive values of 54.81% and 96.77%, and an efficiency of 81.72%. The clinical factors associated with the results were also studied using a univariate and a multivariate analysis, which showed that Scedosporium/Lomentospora positive sputum, elevated anti-Aspergillus serum IgG and chronic Pseudomonas aeruginosa infection were significantly associated with a positive result in DIA, while Staphylococcus aureus positive sputum showed a negative association. In conclusion, the test developed can offer a complementary, rapid, simple and sensitive method to contribute to the diagnosis of Scedosporium/Lomentospora in patients with CF.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectScedosporiumeng
dc.subjectLomentosporaeng
dc.subjectcystic fibriosiseng
dc.subjectimmunoassayeng
dc.subjectserological detectioneng
dc.subjectantibody detectioneng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleDot Immunobinding Assay for the Rapid Serodetection of Scedosporium/Lomentospora in Cystic Fibrosis Patientsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11388-7
dc.type.versionpublishedVersionnone
local.edoc.container-titleJournal of Funginone
local.edoc.container-issn2309-608Xnone
local.edoc.pages16none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/journal/jofnone
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume9none
local.edoc.container-issue2none
local.edoc.container-reportyear2023none
dc.description.versionPeer Reviewednone

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