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2011-03-16Zeitschriftenartikel DOI: 10.1016/j.jviromet.2011.03.013
Joint production of prime/boost pairs of Fowlpox Virus and Modified Vaccinia Ankara recombinants carrying the same transgene
dc.contributor.authorSoprana, Elisa
dc.contributor.authorPanigada, Maddalena
dc.contributor.authorKnauf, Mathias
dc.contributor.authorRadaelli, Antonia
dc.contributor.authorVigevani, Luisa
dc.contributor.authorPalini, Alessio
dc.contributor.authorVilla, Chiara
dc.contributor.authorMalnati, Mauro
dc.contributor.authorCassina, Giulia
dc.contributor.authorKurth, Reinhard
dc.contributor.authorNorley, Stephen
dc.contributor.authorSiccardi, Antonio G.
dc.date.accessioned2018-05-07T15:28:06Z
dc.date.available2018-05-07T15:28:06Z
dc.date.created2012-03-16
dc.date.issued2011-03-16none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rev1kFFuqOsNg/PDF/27XccpgmVhDZc.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1157
dc.description.abstractPairs of recombinant MVA (Modified Vaccinia Ankara) and FPV (Fowlpox Virus) expressing the same transgene are reasonable candidates for prime/boost regimens, because cross-reacting immune responses between the two vectors, both non-replicative in mammalian hosts, are very limited. The acceptor virus FPD-Red, a derivative of FPV, carrying a red fluorescent protein gene flanked by the homology regions of MVA deletion III, was constructed. The same MVA Transfer Plasmid Green, designed to insert transgenes into the MVA deletion III locus, can therefore be used to transfer transgenes into both acceptor viruses MVA-Red and FPD-Red with the described recently Red-to-Green gene swapping method. Cells infected by either recombinant virus can be sorted differentially by a simple and reliable FACS-based purification protocol. The procedure is carried out in primary chick embryo fibroblasts grown in serum-free media and was applied to the production of three rMVA/rFPV pairs expressing the H5N1 avian influenza antigens M1, M2 and NP. The viral genes were human codon-optimized and expressed at high levels in both chick and mammalian cells. Both single-step and multiple-step growth analyses showed no significant differences in growth due to the transgenes in either rMVA or rFPV derivatives.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectCell Lineeng
dc.subjectHumanseng
dc.subjectAnimalseng
dc.subjectInfluenza A Viruseng
dc.subjectViral Proteins/geneticseng
dc.subjectGenetic Vectorseng
dc.subjectVaccinia virus/geneticseng
dc.subjectChickenseng
dc.subjectH5N1 Subtype/geneticseng
dc.subjectRecombinant poxviruseseng
dc.subjectPrime/boost pairseng
dc.subjectDrug Carrierseng
dc.subjectFowlpox virus/geneticseng
dc.subjectGene Therapy/methodseng
dc.subjectTransduction Geneticeng
dc.subjectTransgenes Vaccines Synthetic/geneticseng
dc.subjectViral Vaccines/geneticseng
dc.subject.ddc610 Medizin
dc.titleJoint production of prime/boost pairs of Fowlpox Virus and Modified Vaccinia Ankara recombinants carrying the same transgene
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10021915
dc.identifier.doi10.1016/j.jviromet.2011.03.013
dc.identifier.doihttp://dx.doi.org/10.25646/1082
local.edoc.container-titleJournal of Virological Methods
local.edoc.container-textSoprana, E., Panigada, M., Knauf, M., Radaelli, A., Vigevani, L., Palini, A., Villa, C., Malnati, M., Cassina, G., Kurth, R., Norley, S., Siccardi, A.G. Joint production of prime/boost pairs of Fowlpox Virus and Modified Vaccinia Ankara recombinants carrying the same transgene (2011) Journal of Virological Methods, 174 (1-2), pp. 22-28.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.sciencedirect.com/science/article/pii/S0166093411001030
local.edoc.container-publisher-nameElsevier
local.edoc.container-volume174
local.edoc.container-issue1-2
local.edoc.container-year2011

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