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2021-12-14Zeitschriftenartikel
Insights into the direct anti-influenza virus mode of action of Rhodiola rosea
dc.contributor.authorDöring, Kristin
dc.contributor.authorLangeder, Julia
dc.contributor.authorDuwe, Susanne
dc.contributor.authorTahir, Ammar
dc.contributor.authorGrienke, Ulrike
dc.contributor.authorRollinger, Judith M.
dc.contributor.authorSchmidtke, Michaela
dc.date.accessioned2024-06-10T11:24:28Z
dc.date.available2024-06-10T11:24:28Z
dc.date.issued2021-12-14none
dc.identifier.other1016/j.phymed.2021.153895
dc.identifier.urihttp://edoc.rki.de/176904/11698
dc.description.abstractBackground: The anti-influenza A virus activities and contents of previously isolated most active flavonoids (rhodiosin and tricin) from a standardized hydro-ethanolic R. rosea root and rhizome extract (SHR-5®), did not fully explain the efficacy of SHR-5®. Moreover, the mode of antiviral action of SHR-5® is unknown. Purpose: To determine the anti-influenza viral principle of SHR-5® we evaluated i) the combined anti-influenza virus effect of rhodiosin and tricin, ii) the impact of its tannin-enriched fraction (TE), iii) its antiviral spectrum and mode of action, and iv) its propensity for resistance development in vitro. Methods: The combined anti-influenza virus effect of rhodiosin and tricin and the impact of TE were investigated with cytopathic effect (CPE)-inhibition assays in MDCK cells. A tannin-depleted fraction (TD) and TE were prepared by polyamide column chromatography and dereplicated by LC-MS. Plaque-reduction assays provided insights into the anti-influenza virus profile, the mode of action, and the propensity for resistance development of SHR-5®. Results: Our results i) did not reveal synergistic anti-influenza A virus effects of rhodiosin and tricin, but ii) proved a strong impact of TE mainly composed of prodelphinidin gallate oligomers. iii) TE inhibited the plaque-production of influenza virus A(H1N1)pdm09, A(H3N2), and B (Victoria and Yamagata) isolates (including isolates resistant to neuraminidase and/or M2 ion channel inhibitors) with 50% inhibitory concentration values between 0.12 - 0.53 µg/ml similar to SHR-5®. Mechanistic studies proved a virucidal activity, inhibition of viral adsorption, viral neuraminidase activity, and virus spread by SHR-5® and TE. iv) No resistance development was observed in vitro. Conclusion: For the first time a comprehensive analysis of the anti-influenza virus profile of a hydro-ethanolic R. rosea extract (SHR-5®) was assessed in vitro. The results demonstrating broad-spectrum multiple direct anti-influenza virus activities, and a lack of resistance development to SHR-5® together with its known augmentation of host defense, support its potential role as an adaptogen against influenza virus infection.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY-NC-ND 3.0 DE) Namensnennung - Nicht-kommerziell - Keine Bearbeitung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/de/
dc.subjectroserooteng
dc.subjectnatural producteng
dc.subjectprodelphinidin gallate oligomerseng
dc.subjectacute respiratory infectioneng
dc.subjectinfluenzaeng
dc.subjectresistanceeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleInsights into the direct anti-influenza virus mode of action of Rhodiola roseanone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11698-5
dc.type.versionpublishedVersionnone
local.edoc.container-titlePhytomedicinenone
local.edoc.container-issn0944-7113none
local.edoc.pages9none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.sciencedirect.com/journal/phytomedicinenone
local.edoc.container-publisher-nameElseviernone
local.edoc.container-volume96none
local.edoc.container-reportyear2021none

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