Show simple item record

2021-07-18Zeitschriftenartikel
Real-Time PCR for Molecular Detection of Zoonotic and Non-Zoonotic Giardia spp. in Wild Rodents
dc.contributor.authorKlotz, Christian
dc.contributor.authorRadam, Elke
dc.contributor.authorRausch, Sebastian
dc.contributor.authorGosten-Heinrich, Petra
dc.contributor.authorAebischer, Toni
dc.date.accessioned2024-07-09T14:34:24Z
dc.date.available2024-07-09T14:34:24Z
dc.date.issued2021-07-18none
dc.identifier.other10.3390/microorganisms9081610
dc.identifier.urihttp://edoc.rki.de/176904/11768
dc.description.abstractGiardiasis in humans is a gastrointestinal disease transmitted by the potentially zoonotic Giardia duodenalis genotypes (assemblages) A and B. Small wild rodents such as mice and voles are discussed as potential reservoirs for G. duodenalis but are predominantly populated by the two rodent species Giardia microti and Giardia muris. Currently, the detection of zoonotic and non-zoonotic Giardia species and genotypes in these animals relies on cumbersome PCR and sequencing approaches of genetic marker genes. This hampers the risk assessment of potential zoonotic Giardia transmissions by these animals. Here, we provide a workflow based on newly developed real-time PCR schemes targeting the small ribosomal RNA multi-copy gene locus to distinguish G. muris, G. microti and G. duodenalis infections. For the identification of potentially zoonotic G. duodenalis assemblage types A and B, an established protocol targeting the single-copy gene 4E1-HP was used. The assays were specific for the distinct Giardia species or genotypes and revealed an analytical sensitivity of approximately one or below genome equivalent for the multi-copy gene and of about 10 genome equivalents for the single-copy gene. Retesting a biobank of small rodent samples confirmed the specificity. It further identified the underlying Giardia species in four out of 11 samples that could not be typed before by PCR and sequencing. The newly developed workflow has the potential to facilitate the detection of potentially zoonotic and non-zoonotic Giardia species in wild rodents.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectGiardia spp.eng
dc.subjectwild rodentseng
dc.subjectmolecular detectioneng
dc.subjectqPCReng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleReal-Time PCR for Molecular Detection of Zoonotic and Non-Zoonotic Giardia spp. in Wild Rodentsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11768-6
dc.type.versionpublishedVersionnone
local.edoc.container-titleMicroorganismsnone
local.edoc.container-issn2076-2607none
local.edoc.pages11none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/journal/microorganismsnone
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume9none
local.edoc.container-issue8none
local.edoc.container-reportyear2021none
dc.description.versionPeer Reviewednone

Show simple item record