Show simple item record

2021-04-24Zeitschriftenartikel
Metabolic adaption of Legionella pneumophila during intracellular growth in Acanthamoeba castellanii
dc.contributor.authorKunze, Mareike
dc.contributor.authorSteiner, Thomas
dc.contributor.authorChen, Fan
dc.contributor.authorHuber, Claudia
dc.contributor.authorRydzewski, Kerstin
dc.contributor.authorStämmler, Maren
dc.contributor.authorHeuner, Klaus
dc.contributor.authorEisenreich, Wolfgang
dc.date.accessioned2024-07-10T09:08:41Z
dc.date.available2024-07-10T09:08:41Z
dc.date.issued2021-04-24none
dc.identifier.other10.1016/j.ijmm.2021.151504
dc.identifier.urihttp://edoc.rki.de/176904/11774
dc.description.abstractThe metabolism of Legionella pneumophila strain Paris was elucidated during different time intervals of growth within its natural host Acanthamoeba castellanii. For this purpose, the amoebae were supplied after bacterial infection (t =0 h) with 11 mM [U-13C6]glucose or 3 mM [U-13C3]serine, respectively, during 0 17 h, 17 25 h, or 25 27 h of incubation. At the end of these time intervals, bacterial and amoebal fractions were separated. Each of these fractions was hydrolyzed under acidic conditions. 13C-Enrichments and isotopologue distributions of resulting amino acids and 3-hydroxybutyrate were determined by gas chromatography – mass spectrometry. Comparative analysis of the labelling patterns revealed the substrate preferences, metabolic pathways, and relative carbon fluxes of the intracellular bacteria and their amoebal host during the time course of the infection cycle. Generally, the bacterial infection increased the usage of exogenous glucose via glycolysis by A. castellanii. In contrast, carbon fluxes via the amoebal citrate cycle were not affected. During the whole infection cycle, intracellular L. pneumophila incorporated amino acids from their host into the bacterial proteins. However, partial bacterial de novo biosynthesis from exogenous 13C-Ser and, at minor rates, from 13C-glucose could be shown for bacterial Ala, Asp, Glu, and Gly. More specifically, the catabolic usage of Ser increased during the post- exponential phase of intracellular growth, whereas glucose was utilized by the bacteria throughout the infection cycle and not only late during infection as assumed on the basis of earlier in vitro experiments. The early usage of 13C-glucose by the intracellular bacteria suggests that glucose availability could serve as a trigger for replication of L. pneumophila inside the vacuoles of host cells.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY-NC-ND 3.0 DE) Namensnennung - Nicht-kommerziell - Keine Bearbeitung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/de/
dc.subjectleginella pneumophilaeng
dc.subjectacanthamoeba castelaniieng
dc.subjectpatho-metabolismeng
dc.subjectbipartite metabolismeng
dc.subjectstable isotope labelingeng
dc.subjecthost-pathogen interactioneng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleMetabolic adaption of Legionella pneumophila during intracellular growth in Acanthamoeba castellaniinone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11774-2
dc.type.versionpublishedVersionnone
local.edoc.container-titleInternational Journal of Medical Microbiologynone
local.edoc.container-issn1618-0607none
local.edoc.pages10none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.sciencedirect.com/journal/international-journal-of-medical-microbiologynone
local.edoc.container-publisher-nameElseviernone
local.edoc.container-volume311none
local.edoc.container-issue4none
local.edoc.container-reportyear2021none
dc.description.versionPeer Reviewednone

Show simple item record