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2021-10-24Zeitschriftenartikel
Molecular surveillance and genetic divergence of rotavirus A antigenic epitopes in Gabonese children with acute gastroenteritis
dc.contributor.authorManouana, Gédéon Prince
dc.contributor.authorNiendorf, Sandra
dc.contributor.authorTomazatos, Alexandru
dc.contributor.authorMbong-Ngwese, Mirabeau
dc.contributor.authorNzamba Maloum, Moustapha
dc.contributor.authorNguema-Moure, Paul Alvyn
dc.contributor.authorBingoulou Matsougou, Gedeon
dc.contributor.authorAtegbo, Simon
dc.contributor.authorRossatanga, Elie Gide
dc.contributor.authorBock, Claus-Thomas
dc.contributor.authorBorrmann, Steffen
dc.contributor.authorMordmüller, Benjamin
dc.contributor.authorEibach, Daniel
dc.contributor.authorKremsner, Peter G.
dc.contributor.authorVelavan, Thirumalaisamy P.
dc.contributor.authorAdégnika, Ayôla Akim
dc.date.accessioned2024-07-10T13:24:24Z
dc.date.available2024-07-10T13:24:24Z
dc.date.issued2021-10-24none
dc.identifier.other10.1016/j.ebiom.2021.103648
dc.identifier.urihttp://edoc.rki.de/176904/11784
dc.description.abstractBackground Rotavirus A (RVA) causes acute gastroenteritis in children <5 years of age in sub-Saharan Africa. In this study, we described the epidemiology and genetic diversity of RVA infecting Gabonese children and examined the antigenic variability of circulating strains in relation to available vaccine strains to maximize the public health benefits of introducing rotavirus vaccine through the Expanded Programme on Immunization (EPI) in Gabon. Methods Stool samples were collected consecutively between April 2018 and November 2019 from all hospitalized children <5 years with gastroenteritis and community controls without gastroenteritis. Children were tested for rotavirus A by quantitative RT-PCR and subsequently sequenced to identify circulating rotavirus A genotypes in the most vulnerable population. The VP7 and VP4 (VP8*) antigenic epitopes were mapped to homologs of vaccine strains to assess structural variability and potential impact on antigenicity. Findings Infections were mostly acquired during the dry season. Rotavirus A was detected in 98/177 (55%) hospitalized children with gastroenteritis and 14/67 (21%) of the control children. The most common RVA genotypes were G1 (18%), G3 (12%), G8 (18%), G9 (2%), G12 (25%), with G8 and G9 reported for the first time in Gabon. All were associated either with P[6] (31%) or P[8] (38%) genotypes. Several non-synonymous substitutions were observed in the antigenic epitopes of VP7 (positions 94 and 147) and VP8* (positions 89, 116, 146 and 150), which may modulate the elicited immune responses. Interpretation This study contributes to the epidemiological surveillance of rotavirus A required before the introduction of rotavirus vaccination in the EPI for Gabonese children.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY-NC-ND 3.0 DE) Namensnennung - Nicht-kommerziell - Keine Bearbeitung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/de/
dc.subjectrotavirus aeng
dc.subjectVaccineseng
dc.subjectgenotypeseng
dc.subjectantigenic epitopeseng
dc.subjectacute gastroenteritiseng
dc.subjectAfricaeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleMolecular surveillance and genetic divergence of rotavirus A antigenic epitopes in Gabonese children with acute gastroenteritisnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11784-6
dc.type.versionpublishedVersionnone
local.edoc.container-titleEBioMedicinenone
local.edoc.container-issn2352-3964none
local.edoc.pages12none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.sciencedirect.com/journal/ebiomedicinenone
local.edoc.container-publisher-nameElseviernone
local.edoc.container-volume73none
local.edoc.container-reportyear2021none
dc.description.versionPeer Reviewednone

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