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2021-11-17Zeitschriftenartikel
A Comprehensive Analysis of Human Endogenous Retroviruses HERV-K (HML.2) from Teratocarcinoma Cell Lines and Detection of Viral Cargo in Microvesicles
dc.contributor.authorMorozov, Vladimir A.
dc.contributor.authorMorozov, Alexey V.
dc.date.accessioned2024-07-17T13:20:16Z
dc.date.available2024-07-17T13:20:16Z
dc.date.issued2021-11-17none
dc.identifier.other10.3390/ijms222212398
dc.identifier.urihttp://edoc.rki.de/176904/11796
dc.description.abstractAbout 8% of our genome is composed of sequences from Human Endogenous Retroviruses (HERVs). The HERV-K (HML.2) family, here abbreviated HML.2, is able to produce virus particles that were detected in cell lines, malignant tumors and in autoimmune diseases. Parameters and properties of HML.2 released from teratocarcinoma cell lines GH and Tera-1 were investigated in detail. In most experiments, analyzed viruses were purified by density gradient centrifugation. HML.2 structural proteins, reverse transcriptase (RT) activity, viral RNA (vRNA) and particle morphology were analyzed. The HML.2 markers were predominantly detected in fractions with a buoyant density of 1.16 g/cm3. Deglycosylation of TM revealed truncated forms of transmembrane (TM) protein. Free virions and extracellular vesicles (presumably microvesicles—MVs) with HML.2 elements, including budding intermediates, were detected by electron microscopy. Viral elements and assembled virions captured and exported by MVs can boost specific immune responses and trigger immunomodulation in recipient cells. Sequencing of cDNA clones demonstrated exclusive presence of HERV-K108 env in HML.2 from Tera-1 cells. Not counting two recombinant variants, four known env sequences were found in HML.2 from GH cells. Obtained results shed light on parameters and morphology of HML.2. A possible mechanism of HML.2-induced diseases is discussed.ger
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectteratocarcinomaeng
dc.subjecthuman endogenous retroviruseseng
dc.subjectproteinseng
dc.subjectdeglycosylationeng
dc.subjectelectron microscopyeng
dc.subjectviral RNAeng
dc.subjectRT-PCReng
dc.subjectcloningeng
dc.subjectmicrovesicleseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleA Comprehensive Analysis of Human Endogenous Retroviruses HERV-K (HML.2) from Teratocarcinoma Cell Lines and Detection of Viral Cargo in Microvesiclesnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11796-9
dc.type.versionpublishedVersionnone
local.edoc.container-titleInternational Journal of Molecular Sciencesnone
local.edoc.container-issn1422-0067none
local.edoc.pages23none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/journal/ijmsnone
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume22none
local.edoc.container-issue22none
local.edoc.container-reportyear2021none
dc.description.versionPeer Reviewednone

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