Show simple item record

2021-01-29Zeitschriftenartikel
Ricin Antibodies’ Neutralizing Capacity against Different Ricin Isoforms and Cultivars
dc.contributor.authorOrsini Delgado, Maria Lucia
dc.contributor.authorAvril, Arnaud
dc.contributor.authorPrigent, Julie
dc.contributor.authorDano, Julie
dc.contributor.authorRouaix, Audrey
dc.contributor.authorWorbs, Sylvia
dc.contributor.authorDorner, Brigitte G.
dc.contributor.authorRougeaux, Clémence
dc.contributor.authorBecher, François
dc.contributor.authorFenaille, François
dc.contributor.authorLivet, Sandrine
dc.contributor.authorVolland, Hervé
dc.contributor.authorTournier, Jean-Nicolas
dc.contributor.authorSimon, Stéphanie
dc.date.accessioned2024-07-22T16:25:50Z
dc.date.available2024-07-22T16:25:50Z
dc.date.issued2021-01-29none
dc.identifier.other10.3390/toxins13020100
dc.identifier.urihttp://edoc.rki.de/176904/11806
dc.description.abstractRicin, a highly toxic protein from Ricinus communis, is considered a potential biowarfare agent. Despite the many data available, no specific treatment has yet been approved. Due to their ability to provide immediate protection, antibodies (Abs) are an approach of choice. However, their high specificity might compromise their capacity to protect against the different ricin isoforms (D and E) found in the different cultivars. In previous work, we have shown the neutralizing potential of different Abs (43RCA-G1 (anti ricin A-chain) and RB34 and RB37 (anti ricin B-chain)) against ricin D. In this study, we evaluated their protective capacity against both ricin isoforms. We show that: (i) RB34 and RB37 recognize exclusively ricin D, whereas 43RCA-G1 recognizes both isoforms, (ii) their neutralizing capacity in vitro varies depending on the cultivar, and (iii) there is a synergistic effect when combining RB34 and 43RCA-G1. This effect is also demonstrated in vivo in a mouse model of intranasal intoxication with ricin D/E (1:1), where approximately 60% and 40% of mice treated 0 and 6 h after intoxication, respectively, are protected. Our results highlight the importance of evaluating the effectiveness of the Abs against different ricin isoforms to identify the treatment with the broadest spectrum neutralizing effect.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectricineng
dc.subjectneutralizing antibodieseng
dc.subjectrecombinant antibodieseng
dc.subjectmonoclonal antibodieseng
dc.subjectricin isoformseng
dc.subjectRicinus communis cultivarseng
dc.subjectpulmonary intoxicationeng
dc.subjecttreatmenteng
dc.subjectmouse modeleng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleRicin Antibodies’ Neutralizing Capacity against Different Ricin Isoforms and Cultivarsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11806-3
dc.type.versionpublishedVersionnone
local.edoc.container-titleToxinsnone
local.edoc.container-issn2072-6651none
local.edoc.pages17none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/journal/toxinsnone
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume13none
local.edoc.container-issue2none
local.edoc.container-reportyear2021none
dc.description.versionPeer Reviewednone

Show simple item record