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2021-01-15Zeitschriftenartikel
The Superior Adherence Phenotype of E. coli O104:H4 is Directly Mediated by the Aggregative Adherence Fimbriae Type I
dc.contributor.authorSchiller, Philipp
dc.contributor.authorKnödler, Michael
dc.contributor.authorBerger, Petya
dc.contributor.authorGreune, Lilo
dc.contributor.authorFruth, Angelika
dc.contributor.authorMellmann, Alexander
dc.contributor.authorDersch, Petra
dc.contributor.authorBerger, Michael
dc.contributor.authorDobrindt, Ulrich
dc.date.accessioned2024-07-24T12:44:04Z
dc.date.available2024-07-24T12:44:04Z
dc.date.issued2021-01-15none
dc.identifier.other10.1080/21505594.2020.1868841
dc.identifier.urihttp://edoc.rki.de/176904/11826
dc.description.abstractWhereas the O104:H4 enterohemorrhagic Escherichia coli (EHEC) outbreak strain from 2011 expresses aggregative adherence fimbriae of subtype I (AAF/I), its close relative, the O104:H4 enteroaggregative Escherichia coli (EAEC) strain 55989, encodes AAF of subtype III. Tight adherence mediated by AAF/I in combination with Shiga toxin 2 production has been suggested to result in the outbreak strain’s exceptional pathogenicity. Furthermore, the O104:H4 outbreak strain adheres significantly better to cultured epithelial cells than archetypal EAEC strains expressing different AAF subtypes. To test whether AAF/I expression is associated with the different virulence phenotypes of the outbreak strain, we heterologously expressed AAF subtypes I, III, IV, and V in an AAF-negative EAEC 55989 mutant and compared AAF-mediated phenotypes, incl. autoaggregation, biofilm formation, as well as bacterial adherence to HEp-2 cells. We observed that the expression of all four AAF subtypes promoted bacterial autoaggregation, though with different kinetics. Disturbance of AAF interaction on the bacterial surface via addition of α-AAF antibodies impeded autoaggregation. Biofilm formation was enhanced upon heterologous expression of AAF variants and inversely correlated with the autoaggregation phenotype. Co-cultivation of bacteria expressing different AAF subtypes resulted in mixed bacterial aggregates. Interestingly, bacteria expressing AAF/I formed the largest bacterial clusters on HEp-2 cells, indicating a stronger host cell adherence similar to the EHEC O104:H4 outbreak strain. Our findings show that, compared to the closely related O104:H4 EAEC strain 55989, not only the acquisition of the Shiga toxin phage, but also the acquisition of the AAF/I subtype might have contributed to the increased EHEC O104:H4 pathogenicityeng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectenteroaggregative E. colieng
dc.subjectenterohemorrhagic E. colieng
dc.subjectAAFeng
dc.subjectadherenceeng
dc.subjectbiofilmeng
dc.subjectautoaggregationeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleThe Superior Adherence Phenotype of E. coli O104:H4 is Directly Mediated by the Aggregative Adherence Fimbriae Type Inone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11826-3
dc.type.versionpublishedVersionnone
local.edoc.container-titleVirulencenone
local.edoc.container-issn2150-5608none
local.edoc.pages14none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.tandfonline.com/journals/kvir20none
local.edoc.container-publisher-nameTaylor & Francisnone
local.edoc.container-volume12none
local.edoc.container-issue1none
local.edoc.container-reportyear2021none
local.edoc.container-firstpage346none
local.edoc.container-lastpage359none
dc.description.versionPeer Reviewednone

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