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2021-03-31Zeitschriftenartikel
Detection of a New Resistance-Mediating Plasmid Chimera in a blaOXA-48-Positive Klebsiella pneumoniae Strain at a German University Hospital
dc.contributor.authorSchwanbeck, Julian
dc.contributor.authorBohne, Wolfgang
dc.contributor.authorHasdemir, Ufuk
dc.contributor.authorGroß, Uwe
dc.contributor.authorPfeifer, Yvonne
dc.contributor.authorBunk, Boyke
dc.contributor.authorRiedel, Thomas
dc.contributor.authorSpröer, Cathrin
dc.contributor.authorOvermann, Jörg
dc.contributor.authorFrickmann, Hagen
dc.contributor.authorZautner, Andreas E.
dc.date.accessioned2024-07-24T15:44:33Z
dc.date.available2024-07-24T15:44:33Z
dc.date.issued2021-03-31none
dc.identifier.other10.3390/microorganisms9040720
dc.identifier.urihttp://edoc.rki.de/176904/11829
dc.description.abstractMobile genetic elements, such as plasmids, facilitate the spread of antibiotic resistance genes in Enterobacterales. In line with this, we investigated the plasmid-resistome of seven blaOXA-48 gene-carrying Klebsiella pneumoniae isolates, which were isolated between 2013 and 2014 at the University Medical Center in Göttingen, Germany. All isolates were subjected to complete genome sequencing including the reconstruction of entire plasmid sequences. In addition, phenotypic resistance testing was conducted. The seven isolates comprised both disease-associated isolates and colonizers isolated from five patients. They fell into two clusters of three sequence type (ST)101 and two ST11 isolates, respectively; and ST15 and ST23 singletons. The seven isolates harbored various plasmids of the incompatibility (Inc) groups IncF, IncL/M, IncN, IncR, and a novel plasmid chimera. All blaOXA-48 genes were encoded on the IncL/M plasmids. Of note, distinct phenotypical resistance patterns associated with different sets of resistance genes encoded by IncL/M and IncR plasmids were observed among isolates of the ST101 cluster in spite of high phylogenetic relatedness of the bacterial chromosomes, suggesting nosocomial transmission. This highlights the importance of plasmid uptake and plasmid recombination events for the fast generation of resistance variability after clonal transmission. In conclusion, this study contributes a piece in the puzzle of molecular epidemiology of resistance gene-carrying plasmids in K. pneumoniae in Germany.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectklebsiella pneumoniaeeng
dc.subjectcarbapenem resistanceeng
dc.subjectbeta-lactamaseeng
dc.subjectresistomeeng
dc.subjectplasmideng
dc.subjectphylogenyeng
dc.subjectepidemiologyeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleDetection of a New Resistance-Mediating Plasmid Chimera in a blaOXA-48-Positive Klebsiella pneumoniae Strain at a German University Hospitalnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11829-5
dc.type.versionpublishedVersionnone
local.edoc.container-titleMicroorganismsnone
local.edoc.container-issn2076-2607none
local.edoc.pages23none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/journal/microorganismsnone
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume9none
local.edoc.container-issue4none
dc.description.versionPeer Reviewednone

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