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2021-12-09Zeitschriftenartikel
First Comparative Analysis of Clostridium septicum Genomes Provides Insights Into the Taxonomy, Species Genetic Diversity, and Virulence Related to Gas Gangrene
dc.contributor.authorThomas, Prasad
dc.contributor.authorAbdel-Glil, Mostafa Y.
dc.contributor.authorSubbiaiyan, Anbazhagan
dc.contributor.authorBusch, Anne
dc.contributor.authorEichhorn, Inga
dc.contributor.authorWieler, Lothar H.
dc.contributor.authorNeubauer, Heinrich
dc.contributor.authorPletz, Matthias
dc.contributor.authorSeyboldt, Christian
dc.date.accessioned2024-07-25T10:58:47Z
dc.date.available2024-07-25T10:58:47Z
dc.date.issued2021-12-09none
dc.identifier.other10.3389/fmicb.2021.771945
dc.identifier.urihttp://edoc.rki.de/176904/11841
dc.description.abstractClostridium septicum is a Gram-positive, toxin-producing, and spore-forming bacterium that is recognized, together with C. perfringens, as the most important etiologic agent of progressive gas gangrene. Clostridium septicum infections are almost always fatal in humans and animals. Despite its clinical and agricultural relevance, there is currently limited knowledge of the diversity and genome structure of C. septicum. This study presents the complete genome sequence of C. septicum DSM 7534T type strain as well as the first comparative analysis of five C. septicum genomes. The taxonomy of C. septicum, as revealed by 16S rRNA analysis as well as by genomic wide indices such as protein-based phylogeny, average nucleotide identity, and digital DNA–DNA hybridization indicates a stable clade. The composition and presence of prophages, CRISPR elements and accessory genetic material was variable in the investigated genomes. This is in contrast to the limited genetic variability described for the phylogenetically and phenotypically related species Clostridium chauvoei. The restriction-modification (RM) systems between two C. septicum genomes were heterogeneous for the RM types they encoded. C. septicum has an open pangenome with 2,311 genes representing the core genes and 1,429 accessory genes. The core genome SNP divergence between genome pairs varied up to 4,886 pairwise SNPs. A vast arsenal of potential virulence genes was detected in the genomes studied. Sequence analysis of these genes revealed that sialidase, hemolysin, and collagenase genes are conserved compared to the α-toxin and hyaluronidase genes. In addition, a conserved gene found in all C. septicum genomes was predicted to encode a leucocidin homolog (beta-channel forming cytolysin) similar (71.10% protein identity) to Clostridium chauvoei toxin A (CctA), which is a potent toxin. In conclusion, our results provide first, valuable insights into strain relatedness and genomic plasticity of C. septicum and contribute to our understanding of the virulence mechanisms of this important human and animal pathogen.ger
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectclostridium septicumeng
dc.subjectcomparative analysiseng
dc.subjectgenomeeng
dc.subjectgas gangreneeng
dc.subjectDSM 7534Teng
dc.subjectvirulence factorseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleFirst Comparative Analysis of Clostridium septicum Genomes Provides Insights Into the Taxonomy, Species Genetic Diversity, and Virulence Related to Gas Gangrenenone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11841-8
dc.type.versionpublishedVersionnone
local.edoc.container-titleFrontiers in Microbiologynone
local.edoc.container-issn1664-302Xnone
local.edoc.pages18none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.frontiersin.org/journals/microbiologynone
local.edoc.container-publisher-nameFrontiers Meadia S.A.none
local.edoc.container-volume12none
local.edoc.container-reportyear2021none
dc.description.versionPeer Reviewednone

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