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2021-09-07Zeitschriftenartikel
Functional comparison of MERS-coronavirus lineages reveals increased replicative fitness of the recombinant lineage 5
dc.contributor.authorSchroeder, Simon
dc.contributor.authorChristin, Mache
dc.contributor.authorKleine-Weber, Hannah
dc.contributor.authorCorman, Victor M.
dc.contributor.authorMuth, Doreen
dc.contributor.authorRichter, Anja
dc.contributor.authorFatykhova, Diana
dc.contributor.authorMemish, Ziad A.
dc.contributor.authorStanifer, Megan L.
dc.contributor.authorBoulant, Steeve
dc.contributor.authorGultom, Mitra
dc.contributor.authorDijkman, Ronald
dc.contributor.authorEggeling, Stephan
dc.contributor.authorHocke, Andreas
dc.contributor.authorHippenstiel, Stefan
dc.contributor.authorThiel, Volker
dc.contributor.authorPöhlmann, Stefan
dc.contributor.authorWolff, Thorsten
dc.contributor.authorMüller, Marcel A.
dc.contributor.authorDrosten, Christian
dc.date.accessioned2024-08-26T11:04:39Z
dc.date.available2024-08-26T11:04:39Z
dc.date.issued2021-09-07none
dc.identifier.other10.1038/s41467-021-25519-1
dc.identifier.urihttp://edoc.rki.de/176904/11962
dc.description.abstractMiddle East respiratory syndrome coronavirus (MERS-CoV) is enzootic in dromedary camels across the Middle East and Africa. Virus-induced pneumonia in humans results from animal contact, with a potential for limited onward transmission. Phenotypic changes have been suspected after a novel recombinant clade (lineage 5) caused large nosocomial outbreaks in Saudi Arabia and South Korea in 2016. However, there has been no functional assessment. Here we perform a comprehensive in vitro and ex vivo comparison of viruses from parental and recombinant virus lineages (lineage 3, n = 7; lineage 4, n = 8; lineage 5, n = 9 viruses) from Saudi Arabia, isolated immediately before and after the shift toward lineage 5. Replication of lineage 5 viruses is significantly increased. Transcriptional profiling finds reduced induction of immune genes IFNB1, CCL5, and IFNL1 in lung cells infected with lineage 5 strains. Phenotypic differences may be determined by IFN antagonism based on experiments using IFN receptor knock out and signaling inhibition. Additionally, lineage 5 is more resilient against IFN pre-treatment of Calu-3 cells (ca. 10-fold difference in replication). This phenotypic change associated with lineage 5 has remained undiscovered by viral sequence surveillance, but may be a relevant indicator of pandemic potentialeng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleFunctional comparison of MERS-coronavirus lineages reveals increased replicative fitness of the recombinant lineage 5none
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11962-7
dc.type.versionpublishedVersionnone
local.edoc.container-titleNature Communicationsnone
local.edoc.container-issn2041-1723none
local.edoc.pages13none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.nature.com/ncomms/none
local.edoc.container-publisher-nameSpringer Naturenone
local.edoc.container-volume12none
local.edoc.container-reportyear2021none
dc.description.versionPeer Reviewednone

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