Show simple item record

2021-07-27Zeitschriftenartikel
Quorum sensing governs a transmissive Legionella subpopulation at the pathogen vacuole periphery
dc.contributor.authorStriednig, Bianca
dc.contributor.authorLanner, Ulrike
dc.contributor.authorNiggli, Selina
dc.contributor.authorKatic, Ana
dc.contributor.authorVormittag, Simone
dc.contributor.authorBrülisauer, Sabrina
dc.contributor.authorHochstrasser, Ramon
dc.contributor.authorKaech, Andreas
dc.contributor.authorWelin, Amanda
dc.contributor.authorFlieger, Antje
dc.contributor.authorZiegler, Urs
dc.contributor.authorSchmidt, Alexander
dc.contributor.authorHilbi, Hubert
dc.contributor.authorPersonnic, Nicolas
dc.date.accessioned2024-08-26T12:42:02Z
dc.date.available2024-08-26T12:42:02Z
dc.date.issued2021-07-27none
dc.identifier.other10.15252/embr.202152972
dc.identifier.urihttp://edoc.rki.de/176904/11968
dc.description.abstractThe Gram‐negative bacterium Legionella pneumophila is the causative agent of Legionnaires' disease and replicates in amoebae and macrophages within a distinct compartment, the Legionella‐containing vacuole (LCV). The facultative intracellular pathogen switches between a replicative, non‐virulent and a non‐replicating, virulent/transmissive phase. Here, we show on a single‐cell level that at late stages of infection, individual motile (PflaA‐GFP‐positive) and virulent (PralF‐ and PsidC‐GFP‐positive) L. pneumophila emerge in the cluster of non‐growing bacteria within an LCV. Comparative proteomics of PflaA‐GFP‐positive and PflaA‐GFP‐negative L. pneumophila subpopulations reveals distinct proteomes with flagellar proteins or cell division proteins being preferentially produced by the former or the latter, respectively. Toward the end of an infection cycle (˜ 48 h), the PflaA‐GFP‐positive L. pneumophila subpopulation emerges at the cluster periphery, predominantly escapes the LCV, and spreads from the bursting host cell. These processes are mediated by the Legionella quorum sensing (Lqs) system. Thus, quorum sensing regulates the emergence of a subpopulation of transmissive L. pneumophila at the LCV periphery, and phenotypic heterogeneity underlies the intravacuolar bi‐phasic life cycle of L. pneumophila.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY-NC-ND 3.0 DE) Namensnennung - Nicht-kommerziell - Keine Bearbeitung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/de/
dc.subjectflagellumeng
dc.subjectpathogen vacuoleeng
dc.subjectphenotypic heterogeneityeng
dc.subjectproteomeeng
dc.subjectquorum sensingeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleQuorum sensing governs a transmissive Legionella subpopulation at the pathogen vacuole peripherynone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11968-6
dc.type.versionpublishedVersionnone
local.edoc.container-titleEMBO Reportsnone
local.edoc.container-issn1469-3178none
local.edoc.pages22none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.embopress.org/journal/14693178none
local.edoc.container-publisher-nameEMBO Pressnone
local.edoc.container-volume22none
local.edoc.container-reportyear2021none
dc.description.versionPeer Reviewednone

Show simple item record