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2021-09-21Zeitschriftenartikel
Comparison of MICs in Escherichia coli isolates from human health surveillance with MICs obtained for the same isolates by broth microdilution
dc.contributor.authorSuwono, Beneditta
dc.contributor.authorHammerl, Jens André
dc.contributor.authorEckmanns, Tim
dc.contributor.authorRoswitha, Merle
dc.contributor.authorEigner, Ulrich
dc.contributor.authorLümen, Michaela
dc.contributor.authorLauter, Sven
dc.contributor.authorStock, Rüdiger
dc.contributor.authorFenner, Ines
dc.contributor.authorBoemke, Eva
dc.contributor.authorTenhagen, Bernd-Alois
dc.date.accessioned2024-08-26T12:56:44Z
dc.date.available2024-08-26T12:56:44Z
dc.date.issued2021-09-21none
dc.identifier.other10.1093/jacamr/dlab145
dc.identifier.urihttp://edoc.rki.de/176904/11969
dc.description.abstractObjectives Human health surveillance and food safety monitoring systems use different antimicrobial susceptibility testing (AST) methods. In this study, we compared the MICs of Escherichia coli isolates provided by these methods. Methods E. coli isolates (n = 120) from human urine samples and their MICs were collected from six medical laboratories that used automated AST methods based on bacterial growth kinetic analyses. These isolates were retested using broth microdilution, which is used by the food safety monitoring system. The essential and categorical agreements (EA and CA), very major errors (VME), major errors (ME) and minor errors (mE) for these two methods were calculated for 11 antibiotics using broth microdilution as a reference. For statistical analysis, clinical breakpoints provided by EUCAST were used. Results Five study laboratories used VITEK®2 and one MicroScan (Walkaway Combo Panel). Out of 120 isolates, 118 isolates (98.3%) were confirmed as E. coli. The 99 E. coli isolates from five study laboratories that used VITEK®2 showed high proportions of EA and CA with full agreements for gentamicin, meropenem, imipenem and ertapenem. Additionally, 100% CA was also observed in cefepime. Few VME (0.5%), ME (1.9%) and mE (1.5%) were observed across all antibiotics. One VME for ceftazidime (7.1%) and 12 MEs for ampicillin (29.4%), cefotaxime (2.4%), ciprofloxacin (3.2%), tigecycline (1.5%) and trimethoprim (22.2%) were detected. Conclusions MICs from E. coli isolates produced by VITEK®2 were similar to those determined by broth microdilution. These results will be valuable for comparative analyses of resistance data from human health surveillance and food safety monitoring systems.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY-NC 3.0 DE) Namensnennung - Nicht kommerziell 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/de/
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleComparison of MICs in Escherichia coli isolates from human health surveillance with MICs obtained for the same isolates by broth microdilutionnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11969-8
dc.type.versionpublishedVersionnone
local.edoc.container-titleJAC - Antimicrobial Resistancenone
local.edoc.container-issn2632-1823none
local.edoc.pages4none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://academic.oup.com/jacamrnone
local.edoc.container-publisher-nameOxford University Pressnone
local.edoc.container-volume3none
local.edoc.container-issue3none
local.edoc.container-reportyear2021none
dc.description.versionPeer Reviewednone

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