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2021-12-15Zeitschriftenartikel
Critical Assessment of MetaProteome Investigation (CAMPI): a multi-laboratory comparison of established workflows
dc.contributor.authorvan den Bossche, Tim
dc.contributor.authorKunath, Benoit J.
dc.contributor.authorSchallert, Kay
dc.contributor.authorSchäpe, Stephanie S.
dc.contributor.authorAbraham, Paul E.
dc.contributor.authorArmengaud, Jean
dc.contributor.authorArntzen, Magnus Ø.
dc.contributor.authorBassignani, Ariane
dc.contributor.authorBenndorf, Dirk
dc.contributor.authorfuchs, Stephan
dc.contributor.authorGiannone, Richard J.
dc.contributor.authorGriffin, Timothy J.
dc.contributor.authorHagen, Live H.
dc.contributor.authorHalder, Rashi
dc.contributor.authorHenry, Céline
dc.contributor.authorHettich, Robert L.
dc.contributor.authorHeyer, Robert
dc.contributor.authorJagtap, Patrik
dc.contributor.authorJehmlich, Nico
dc.contributor.authorJensen, Marlene
dc.contributor.authorJuste, Catherine
dc.contributor.authorKleiner, Manuel
dc.contributor.authorLangella, Olivier
dc.contributor.authorLehmann, Theresa
dc.contributor.authorLeith, Emma
dc.contributor.authorMay, Patrick
dc.contributor.authorMesuere, Patrick
dc.contributor.authorMiotello, Guylaine
dc.contributor.authorPeters, Samantha L.
dc.contributor.authorPible, Olivier
dc.contributor.authorQueiros, Pedro T.
dc.contributor.authorReichl, Udo
dc.contributor.authorRenard, Bernhard Y.
dc.contributor.authorSchiebenhoefer, Henning
dc.contributor.authorSczyrba, Alexander
dc.contributor.authorTanca, Allesandro
dc.contributor.authorTrappe, Kathrin
dc.contributor.authorTrezzi, Jean-Pierre
dc.contributor.authorUzzau, Sergio
dc.contributor.authorVerschaffelt, Pieter
dc.contributor.authorvon Bergen, Martin
dc.contributor.authorWilmes, Paul
dc.contributor.authorWolf, Maximilian
dc.contributor.authorMartens, Lennart
dc.contributor.authorMuth, Thilo
dc.date.accessioned2024-08-26T15:27:17Z
dc.date.available2024-08-26T15:27:17Z
dc.date.issued2021-12-15none
dc.identifier.other10.1038/s41467-021-27542-8
dc.identifier.urihttp://edoc.rki.de/176904/11976
dc.description.abstractMetaproteomics has matured into a powerful tool to assess functional interactions in microbial communities. While many metaproteomic workflows are available, the impact of method choice on results remains unclear. Here, we carry out a community-driven, multi-laboratory comparison in metaproteomics: the critical assessment of metaproteome investigation study (CAMPI). Based on well-established workflows, we evaluate the effect of sample preparation, mass spectrometry, and bioinformatic analysis using two samples: a simplified, laboratory-assembled human intestinal model and a human fecal sample. We observe that variability at the peptide level is predominantly due to sample processing workflows, with a smaller contribution of bioinformatic pipelines. These peptide-level differences largely disappear at the protein group level. While differences are observed for predicted community composition, similar functional profiles are obtained across workflows. CAMPI demonstrates the robustness of present-day metaproteomics research, serves as a template for multi-laboratory studies in metaproteomics, and provides publicly available data sets for benchmarking future developments.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleCritical Assessment of MetaProteome Investigation (CAMPI): a multi-laboratory comparison of established workflowsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11976-1
dc.type.versionpublishedVersionnone
local.edoc.container-titleNature Communicationsnone
local.edoc.container-issn2041-1723none
local.edoc.pages15none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.nature.com/ncomms/none
local.edoc.container-publisher-nameSpringer Naturenone
local.edoc.container-volume12none
local.edoc.container-reportyear2021none
dc.description.versionPeer Reviewednone

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