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2022-07-06Zeitschriftenartikel
Genetic diversity and spatial distribution of Burkholderia mallei by core genome-based multilocus sequence typing analysis
dc.contributor.authorAppelt, Sandra
dc.contributor.authorRohleder, Anna-Maria
dc.contributor.authorJacob, Daniel
dc.contributor.authorvon Buttlar, Heiner
dc.contributor.authorGeorgi, Enrico
dc.contributor.authorMueller, Katharina
dc.contributor.authorWernery, Ulrich
dc.contributor.authorKinne, Joerg
dc.contributor.authorJoseph, Marina
dc.contributor.authorJose, Shantymol V.
dc.contributor.authorScholz, Holger C.
dc.date.accessioned2024-08-27T13:03:21Z
dc.date.available2024-08-27T13:03:21Z
dc.date.issued2022-07-06none
dc.identifier.other10.1371/journal.pone.0270499
dc.identifier.urihttp://edoc.rki.de/176904/11989
dc.description.abstractBurkholderia mallei is the etiological agent of glanders, a highly contagious and often fatal disease in equids. Due to the high genetic clonality of B. mallei, high-resolution typing assays are necessary to differentiate between individual strains. Here we report on the development and validation of a robust and reproducible core genome-based Multi Locus Sequence Typing Assay (cgMLST) for B. mallei, which is based on 3328 gene targets and enables high-resolution typing at the strain level. The assay was validated using a set of 120 B. mallei genomes from public databases and 23 newly sequenced outbreak strains from in-house strain collections. In this cgMLST analysis, strains from different geographic regions were clearly distinguished by at least 70 allele differences, allowing spatial clustering while closely related and epidemiologically related strains were separated by only zero to three alleles. Neither the different sequencing technologies nor the assembly strategies had an influence on the cgMLST results. The developed cgMLST is highly robust, reproducible and can be used for outbreak investigations, source tracking and molecular characterization of new B. mallei isolates.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleGenetic diversity and spatial distribution of Burkholderia mallei by core genome-based multilocus sequence typing analysisnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/11989-7
dc.type.versionpublishedVersionnone
local.edoc.container-titlePLOS ONEnone
local.edoc.container-issn1932-6203none
local.edoc.pages18none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://journals.plos.org/plosone/none
local.edoc.container-publisher-namePLOSnone
local.edoc.container-volume17none
local.edoc.container-issue7none
local.edoc.container-reportyear2022none
dc.description.versionPeer Reviewednone

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