Show simple item record

2022-12-13Zeitschriftenartikel
Evaluation of CHROMagar Acinetobacter and MacConkey media for the recovery of Acinetobacter baumannii from soil samples
dc.contributor.authorYusuf, Ibrahim
dc.contributor.authorSkiebe, Evelyn
dc.contributor.authorWilharm, Gottfried
dc.date.accessioned2024-08-29T09:23:47Z
dc.date.available2024-08-29T09:23:47Z
dc.date.issued2022-12-13none
dc.identifier.other10.1093/lambio/ovac051
dc.identifier.urihttp://edoc.rki.de/176904/12029
dc.description.abstractThe opportunistic pathogen Acinetobacter baumannii occupies niches in human and veterinary clinics and other environments. This comparative study was designed to assess the recovery rate of Ac. baumannii from 20 soil samples collected in Germany (from which Ac. baumannii had been previously isolated) on selective CHROMagar Acinetobacter (CMA) and MacConkey Agar (MCA). Suspected Ac. baumannii were confirmed by detection and sequencing of blaOXA-51-like genes. Overall, the recovery rate of Ac. baumannii from both media was similar. Out of the 20 soil samples enriched in mineral salt medium (MM) plus 0.2% acetate for 5 h, Ac. baumannii was recovered from 12 (60%) on CMA and 11 (55%) on MCA, and from 14 (70%) with both selective media after enrichment for 24 h. Typical and distinct colonies of Ac. baumannii were more often observed on CMA with soil enriched in MM for 5 h, while on MCA distinct colonies were more obvious after 24 h. In five soil samples (25%), strains harbouring different OXA-51-like variants were recovered on MCA. Late lactose fermentation (48 h) was observed on MCA. The study suggests that no single growth medium would efficiently recover Ac. baumannii from all soil samples.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY-NC 3.0 DE) Namensnennung - Nicht kommerziell 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/de/
dc.subjectacinetobacter baumanniieng
dc.subjectsoileng
dc.subjectMacCokey agareng
dc.subjectCHROMagareng
dc.subjectacinetobactereng
dc.subjectculture mediaeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleEvaluation of CHROMagar Acinetobacter and MacConkey media for the recovery of Acinetobacter baumannii from soil samplesnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/12029-3
dc.type.versionpublishedVersionnone
local.edoc.container-titleLetters in Applied Microbiologynone
local.edoc.container-issn1472-765Xnone
local.edoc.pages7none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://academic.oup.com/lambionone
local.edoc.container-publisher-nameOxford University Pressnone
local.edoc.container-volume76none
local.edoc.container-issue2none
local.edoc.container-reportyear2022none
dc.description.versionPeer Reviewednone

Show simple item record