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2022-08-08Zeitschriftenartikel
Molecular Epidemiology of Mycobacterium abscessus Isolates Recovered from German Cystic Fibrosis Patients
dc.contributor.authorWetzstein, Nils
dc.contributor.authorDiricks, Margo
dc.contributor.authorKohl, Thomas A.
dc.contributor.authorWichelhaus, Thomas A.
dc.contributor.authorAndres, Sönke
dc.contributor.authorPaulowski, Laura
dc.contributor.authorSchwarz, Carsten
dc.contributor.authorLewin, Astrid
dc.contributor.authorKehrmann, Jan
dc.contributor.authorKahl, Barbara C.
dc.contributor.authorDichtl, Karl
dc.contributor.authorHügel, Christian
dc.contributor.authorEickmeier, Olaf
dc.contributor.authorSmaczny, Christina
dc.contributor.authorSchmidt, Annika
dc.contributor.authorZimmermann, Stefan
dc.contributor.authorNährlich, Lutz
dc.contributor.authorHafkemeyer, Sylvia
dc.contributor.authorNiemann, Stefan
dc.contributor.authorMaurer, Florian P.
dc.contributor.authorHogardt, Michael
dc.date.accessioned2024-08-29T13:23:23Z
dc.date.available2024-08-29T13:23:23Z
dc.date.issued2022-08-08none
dc.identifier.other10.1128/spectrum.01714-22
dc.identifier.urihttp://edoc.rki.de/176904/12035
dc.description.abstractInfections due to Mycobacterium abscessus are a major cause of mortality and morbidity in cystic fibrosis (CF) patients. Furthermore, M. abscessus has been suspected to be involved in person-to-person transmissions. In 2016, dominant global clonal complexes (DCCs) that occur worldwide among CF patients have been described. To elucidate the epidemiological situation of M. abscessus among CF patients in Germany and to put these data into a global context, we performed whole-genome sequencing of a set of 154 M. abscessus isolates from 123 German patients treated in 14 CF centers. We used MTBseq pipeline to identify clusters of closely related isolates and correlate those with global findings. Genotypic drug susceptibility for macrolides and aminoglycosides was assessed by characterization of the erm(41), rrl, and rrs genes. By this approach, we could identify representatives of all major DCCs (Absc 1, Absc 2, and Mass 1) in our cohort. Intrapersonal isolates showed higher genetic relatedness than interpersonal isolates (median 3 SNPs versus 16 SNPs; P < 0.001). We further identified four clusters with German patients from same centers clustering with less than 25 SNPs distance (range 3 to 18 SNPs) but did not find any hint for in-hospital person-to-person transmission. This is the largest study investigating phylogenetic relations of M. abscessus isolates in Germany. We identified representatives of all reported DCCs but evidence for nosocomial transmission remained inconclusive. Thus, the occurrence of genetically closely related isolates of M. abscessus has to be interpreted with care, as a direct interhuman transmission cannot be directly deduced.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectmycobacterium abscessuseng
dc.subjectcystic fibrosiseng
dc.subjectwhole-genome sequencingeng
dc.subjectdominant circulating cloneseng
dc.subjecthospital transmissioneng
dc.subjectGerman CF registryeng
dc.subjectnontuberculous mycobacteriaeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleMolecular Epidemiology of Mycobacterium abscessus Isolates Recovered from German Cystic Fibrosis Patientsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/12035-1
dc.type.versionpublishedVersionnone
local.edoc.container-titleMicrobiology Spectrumnone
local.edoc.container-issn2165-0497none
local.edoc.pages11none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://journals.asm.org/journal/spectrumnone
local.edoc.container-publisher-nameAmerican Society for Microbiologynone
local.edoc.container-volume10none
local.edoc.container-issue4none
local.edoc.container-reportyear2022none
dc.description.versionPeer Reviewednone

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