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2022Zeitschriftenartikel
Metabolic plasticity of Francisella tularensis subsp. holarctica (wild type), Francisella novicida and Francisella sp. strain W12-1067
dc.contributor.authorSteiner, Thomas
dc.contributor.authorChen, Fan
dc.contributor.authorRydzewski, Kerstin
dc.contributor.authorMorguet, Clara
dc.contributor.authorAchatz, Felicia
dc.contributor.authorEisenreich, Wolfgang
dc.contributor.authorHeuner, Klaus
dc.date.accessioned2024-09-02T13:02:24Z
dc.date.available2024-09-02T13:02:24Z
dc.date.issued2022none
dc.identifier.other10.51585/gjm.2022.1.0012
dc.identifier.urihttp://edoc.rki.de/176904/12062
dc.description.abstractFrancisella tularensis is a Gram-negative bacterium that causes a potentially fatal disease called tularemia. The highly infectious agent can spread via arthropod vectors, including ticks, and via rodents such as rabbits or beavers. The facultative intracellular pathogen typically invades human macrophages at the onset of human infection. For intracellular replication of the bacteria, the usage of amino acids from the host cells and gluconeogenesis seem to be detrimental, but other carbon sources including glucose and glycerol are also utilized. Here, we compared the growth phase-dependent degradation of glucose, glycerol, and alanine in F. tularensis subsp. holarctica isolated from an infected beaver with the respective metabolism in the less virulent strains F. novicida strain U112 and Francisella sp. strain W12-1067. To this aim, we performed 13C-labeling experiments with the bacteria growing in medium T supplemented with either [U-13C6]glucose, [U-13C3]glycerol, or [2,3-13C2]alanine during different growth phases. After cell harvest, mechanical disruption, and hydrolysis of cellular fractions, we determined the 13C-profiles in various metabolites by mass spectrometry. The detected 13C-patterns elucidated the metabolic fate of the supplied carbon nutrients and revealed minor, but significant differences indicative of various metabolic phenotypes of the Francisella strains under study. Glucose served as the main substrate for all strains under the experimental conditions. The sugar was degraded via the Embden-Meyerhof-Parnas pathway as the major catabolic route during growth. At lower rates, exogenous glycerol and alanine were used as co-substrates, particularly in the less pathogenic strains during the later growth phases. Our data support the hypothesis that, among other factors, the capability to adapt substrate usages efficiently and metabolic fluxes could determine the virulence of Francisella strains.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY-NC 3.0 DE) Namensnennung - Nicht kommerziell 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/de/
dc.subjectfrancisellaeng
dc.subjectintracellular bacteriaeng
dc.subject13C-Labelingeng
dc.subjectisotopologue profilingeng
dc.subjectmetabolic adaptationeng
dc.subjecttularemiaeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleMetabolic plasticity of Francisella tularensis subsp. holarctica (wild type), Francisella novicida and Francisella sp. strain W12-1067none
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/12062-1
dc.type.versionpublishedVersionnone
local.edoc.container-titleGerman Journal of Microbiologynone
local.edoc.container-issn2749-0149none
local.edoc.pages22none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://gmpc-akademie.de/journals/gjmnone
local.edoc.container-publisher-nameGerman Multidisciplinary Publishing Centernone
local.edoc.container-volume2none
local.edoc.container-issue1none
local.edoc.container-reportyear2022none
local.edoc.container-firstpage19none
local.edoc.container-lastpage29none
dc.description.versionPeer Reviewednone

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