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2022-11-22Zeitschriftenartikel
The BDSF quorum sensing receptor RpfR regulates Bep exopolysaccharide synthesis in Burkholderia cenocepacia via interaction with the transcriptional regulator BerB
dc.contributor.authorSteiner, Elisabeth
dc.contributor.authorShilling, Rebecca E.
dc.contributor.authorRichter, Anja M.
dc.contributor.authorSchmid, Nadine
dc.contributor.authorFazli, Mustafa
dc.contributor.authorKaever, Volkhard
dc.contributor.authorJenal, Urs
dc.contributor.authorTolker-Nielsen, Tim
dc.contributor.authorEberl, Leo
dc.date.accessioned2024-09-02T14:40:58Z
dc.date.available2024-09-02T14:40:58Z
dc.date.issued2022-11-22none
dc.identifier.other10.1038/s41522-022-00356-2
dc.identifier.urihttp://edoc.rki.de/176904/12063
dc.description.abstractThe polysaccharide Bep is essential for in vitro biofilm formation of the opportunistic pathogen Burkholderia cenocepacia. We found that the Burkholderia diffusible signaling factor (BDSF) quorum sensing receptor RpfR is a negative regulator of the bep gene cluster in B. cenocepacia. An rpfR mutant formed wrinkled colonies, whereas additional mutations in the bep genes or known bep regulators like berA and berB restored the wild-type smooth colony morphology. We found that there is a good correlation between intracellular c-di-GMP levels and bep expression when the c-di-GMP level is increased or decreased through ectopic expression of a diguanylate cyclase or a c-di-GMP phosphodiesterase, respectively. However, when the intracellular c-di-GMP level is changed by site directed mutagenesis of the EAL or GGDEF domain of RpfR there is no correlation between intracellular c-di-GMP levels and bep expression. Except for rpfR, deletion mutants of all 25 c-di-GMP phosphodiesterase and diguanylate cyclase genes encoded by B. cenocepacia showed no change to berA and bep gene expression. Moreover, bacterial two-hybrid assays provided evidence that RpfR and BerB physically interact and give specificity to the regulation of the bep genes. We suggest a model where RpfR binds BerB at low c-di-GMP levels to sequester this RpoN-dependent activator to an RpfR/RpfF complex. If the c-di-GMP levels rise, possibly by the enzymatic action of RpfR, BerB binds c-di-GMP and is released from the RpfR/RpfF complex and associates with RpoN to activate transcription of berA, and the BerA protein subsequently activates transcription of the bep genes.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleThe BDSF quorum sensing receptor RpfR regulates Bep exopolysaccharide synthesis in Burkholderia cenocepacia via interaction with the transcriptional regulator BerBnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/12063-6
dc.type.versionpublishedVersionnone
local.edoc.container-titlenpj biofilms and microbiomesnone
local.edoc.container-issn2005-5008none
local.edoc.pages13none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.nature.com/npjbiofilms/none
local.edoc.container-publisher-nameSpringer Naturenone
local.edoc.container-volume8none
local.edoc.container-issue93none
local.edoc.container-reportyear2022none
dc.description.versionPeer Reviewednone

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