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2022-10-20Zeitschriftenartikel
Water flow through bone: Neutron tomography reveals differences in water permeability between osteocytic and anosteocytic bone material
dc.contributor.authorSilveira, Andreia
dc.contributor.authorKardjilov, Nikolay
dc.contributor.authorMarkötter, Henning
dc.contributor.authorLongo, Elena
dc.contributor.authorGreving, Imke
dc.contributor.authorLasch, Peter
dc.contributor.authorShahar, Ron
dc.contributor.authorZaslansky, Paul
dc.date.accessioned2024-09-02T15:56:48Z
dc.date.available2024-09-02T15:56:48Z
dc.date.issued2022-10-20none
dc.identifier.other10.1016/j.matdes.2022.111275
dc.identifier.urihttp://edoc.rki.de/176904/12071
dc.description.abstractVertebrate bones are made of a nanocomposite consisting of water, mineral and organics. Water helps bone material withstand mechanical stress and participates in sensation of external loads. Water diffusion across vertebrae of medaka (bone material lacking osteocytes) and zebrafish (bone material containing osteocytes) was compared using neutron tomography. Samples were measured both wet and following immersion in deuterated-water (D2O). By quantifying H+ exchange and mutual alignment with X-ray µCT scans, the amount of water expelled from complete vertebra was determined. The findings revealed that anosteocytic bone material is almost twice as amenable to D2O diffusion and H2O exchange, and that unexpectedly, far more water is retained in osteocytic zebrafish bone. Diffusion in osteocytic bones (only 33 % – 39 % water expelled) is therefore restricted as compared to anosteocytic bone (∼ 60 % of water expelled), presumably because water flow is confined to the lacunar-canalicular network (LCN) open-pore system. Histology and Raman spectroscopy showed that anosteocytic bone contains less proteoglycans than osteocytic bone. These findings identify a previously unknown functional difference between the two bone materials. Therefore, this study proposes that osteocytic bone retains water, aided by non-collagenous proteins, which contribute to its poroelastic mechano-transduction of water flow confined inside the LCN porosity.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY-NC-ND 3.0 DE) Namensnennung - Nicht-kommerziell - Keine Bearbeitung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/de/
dc.subjectbone porosityeng
dc.subjectanosteocytic boneeng
dc.subjectwater permeabilityeng
dc.subjectneutron tomographyeng
dc.subjectproteoglycanseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleWater flow through bone: Neutron tomography reveals differences in water permeability between osteocytic and anosteocytic bone materialnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/12071-1
dc.type.versionpublishedVersionnone
local.edoc.container-titleMaterials &Designnone
local.edoc.container-issn1873-4197none
local.edoc.pages13none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.sciencedirect.com/journal/materials-and-designnone
local.edoc.container-publisher-nameElseviernone
local.edoc.container-volume224none
local.edoc.container-issueDecember 2022none
local.edoc.container-reportyear2022none
dc.description.versionPeer Reviewednone

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