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2022-07-27Zeitschriftenartikel
Few Amino Acid Mutations in H6 Influenza A Virus From South American Lineage Increase Viral Replication Efficiency in Poultry
dc.contributor.authorRimondi, Augustina
dc.contributor.authorOlivera, Valeria S.
dc.contributor.authorSoria, Ivana
dc.contributor.authorParisi, Gustavo D.
dc.contributor.authorRumbo, Martin
dc.contributor.authorPerez, Daniel R.
dc.date.accessioned2024-09-03T16:30:24Z
dc.date.available2024-09-03T16:30:24Z
dc.date.issued2022-07-27none
dc.identifier.other10.3389/fmicb.2022.953738
dc.identifier.urihttp://edoc.rki.de/176904/12084
dc.description.abstractIn chickens, infections due to influenza A virus (IAV) can be mild to severe and lethal. The study of IAV infections in poultry has been mostly limited to strains from the North American and Eurasian lineages, whereas limited information exists on similar studies with strains from the South American lineage (SAm). To better evaluate the risk of introduction of a prototypical SAm IAV strain into poultry, chickens were infected with a wild-type SAm origin strain (WT557/H6N2). The resulting virus progeny was serially passaged in chickens 20 times, and the immunopathological effects of the last passage virus, 20Ch557/H6N2, in chickens were compared to those of the parental strain. A comparison of complete viral genome sequences indicated that the 20Ch557/H6N2 strain contained 13 amino acid differences compared to the wild-type strain. Five of these mutations are in functionally relevant regions of the viral surface glycoproteins hemagglutinin (HA) and neuraminidase (NA). However, despite higher and more prolonged virus shedding in chickens inoculated with the 20Ch557/H6N2 strain compared to those that received the WT557/H6N2 strain, transmission to naïve chickens was not observed for either group. Analyses by flow cytometry of mononuclear cells and lymphocyte subpopulations from the lamina propria and intraepithelial lymphocytic cells (IELs) from the ileum revealed a significant increase in the percentages of CD3+TCRγδ+ IELs in chickens inoculated with the 20Ch557/H6N2 strain compared to those inoculated with the WT557/H6N2 strain.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectinfluenza A viruseng
dc.subjectSouth American IAVeng
dc.subjectviral adaptationeng
dc.subjectviral fitnesseng
dc.subjectviral transmissioneng
dc.subjectchicken studieseng
dc.subjectrisk to poultryeng
dc.subjectimmunopathogenesiseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleFew Amino Acid Mutations in H6 Influenza A Virus From South American Lineage Increase Viral Replication Efficiency in Poultrynone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/12084-9
dc.type.versionpublishedVersionnone
local.edoc.container-titleFrontiers in Microbiologynone
local.edoc.container-issn1664-302Xnone
local.edoc.pages10none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.frontiersin.org/journals/microbiologynone
local.edoc.container-publisher-nameFrontiers Meadia S.A.none
local.edoc.container-volume13none
local.edoc.container-reportyear2022none
dc.description.versionPeer Reviewednone

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