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2022-06-29Zeitschriftenartikel
First Complete Cytochrome B Sequences and Molecular Taxonomy of Bat Species from Sri Lanka
dc.contributor.authorPerera, Thejanee
dc.contributor.authorSchwarz, Franziska
dc.contributor.authorMuzeniek, Therese
dc.contributor.authorSiriwardana, Sahan
dc.contributor.authorBecker-Ziaja, Beate
dc.contributor.authorPerera, Inoka C.
dc.contributor.authorHandunetti, Shiroma
dc.contributor.authorWeerasena, Jagathpriya
dc.contributor.authorPremawansa, Gayani
dc.contributor.authorPremawansa, Sunil
dc.contributor.authorNitsche, Andreas
dc.contributor.authorYapa, Wipula
dc.contributor.authorKohl, Claudia
dc.date.accessioned2024-09-05T09:57:15Z
dc.date.available2024-09-05T09:57:15Z
dc.date.issued2022-06-29none
dc.identifier.other10.3390/ani12131674
dc.identifier.urihttp://edoc.rki.de/176904/12108
dc.description.abstractThe aim of our study was to address the research gap in the molecular taxonomy of Sri Lankan bats. The accurate identification of animals plays a major role in observing them in their natural environments and hence understanding possible disease-transmitting pathways from animals to humans. Being a tropical country, Sri Lanka has a high density of animals. There are 30 different species of bats described in Sri Lanka. Until now, the animals have been identified by observing their physical features. However, the visual identification of animals is not accurate because closely related animal groups may show similar physical features. During our study, we accurately differentiated five bat groups living in one of the largest caves in Sri Lanka by using a more sophisticated laboratory technique. Using molecular techniques, we were able to provide more accurate results than by the visual identification of the bats. The results from our study are stored in the NCBI database as a baseline for a repository of Sri Lankan bats. With the new sequence data provided here, we filled the gap concerning the molecular taxonomy of bat species of the entire region and we contributed to the future conservation and systematic studies of these mammalseng
dc.description.abstractThis is the first report on the molecular identification and phylogeny of the Rousettus leschenaultii Desmarest, 1810, Rhinolophus rouxii Temminck, 1835, Hipposideros speoris Schneider, 1800, Hipposideros lankadiva Kelaart, 1850, and Miniopterus fuliginosus Kuhl, 1817, bat species in Sri Lanka, inferred from analyses by mitochondrially encoded cytochrome b gene sequences. Recent research has indicated that bats show enormous cryptic genetic diversity. Moreover, even within the same species, the acoustic properties of echolocation calls and morphological features such as fur color could vary in different populations. Therefore, we have used molecular taxonomy for the accurate identification of five bat species recorded in one of the largest cave populations in Sri Lanka. The bats were caught using a hand net, and saliva samples were collected non-invasively from each bat by using a sterile oral swab. Nucleic acids were extracted from the oral swab samples, and mitochondrial DNA was amplified by using primers targeting the mitochondrially encoded cytochrome b gene. This study reports the first molecular evidence for the identification of five bat species in Sri Lanka. Our findings will contribute to future conservation and systematic studies of bats in Sri Lanka. This study will also provide the basis for a genetic database of Sri Lankan bats which will contribute significantly to the investigation of potentially zoonotic bat viruses.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectmolecular identification of batseng
dc.subjectbat phylogenyeng
dc.subjectSri Lankan batseng
dc.subjectmolecular phylogenyeng
dc.subjectOne Healtheng
dc.subjectmolecular taxonomyeng
dc.subjectcryptic specieseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleFirst Complete Cytochrome B Sequences and Molecular Taxonomy of Bat Species from Sri Lankanone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/12108-6
dc.type.versionpublishedVersionnone
local.edoc.container-titleanimalsnone
local.edoc.container-issn2076-2615none
local.edoc.pages8none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/journal/animalsnone
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume12none
local.edoc.container-issue13none
local.edoc.container-reportyear2022none
dc.description.versionPeer Reviewednone

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