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2022-08-13Zeitschriftenartikel
Efficacy of Off-Label Anti-Amoebic Agents to Suppress Trophozoite Formation of Acanthamoeba spp. on Non-Nutrient Agar Escherichia Coli Plates
dc.contributor.authorMuthukumar, Vithusan
dc.contributor.authorShi, Lei
dc.contributor.authorChai, Ning
dc.contributor.authorLangenbucher, Achim
dc.contributor.authorBecker, Sören L.
dc.contributor.authorSeitz, Berthold
dc.contributor.authorOrosz, Erika
dc.contributor.authorStachon, Tanja
dc.contributor.authorKiderlen, Albrecht F.
dc.contributor.authorBischoff, Markus
dc.contributor.authorSzentmáry, Nóra
dc.date.accessioned2024-09-09T16:13:56Z
dc.date.available2024-09-09T16:13:56Z
dc.date.issued2022-08-13none
dc.identifier.other10.3390/microorganisms10081642
dc.identifier.urihttp://edoc.rki.de/176904/12121
dc.description.abstractAcanthamoeba keratitis (AK) is a dangerous infectious disease, which is associated with a high risk of blindness for the infected patient, and for which no standard therapy exists thus far. Patients suffering from AK are thus treated, out of necessity, with an off-label therapy, using drugs designed and indicated for other diseases/purposes. Here, we tested the capability of the off-label anti-amoebic drugs chlorhexidine (CH; 0.1%), dibromopropamidine diisethionate (DD; 0.1%), hexamidine diisethionate (HD; 0.1%), miltefosine (MF; 0.0065%), natamycin (NM; 5%), polyhexamethylene biguanide (PHMB; 0.02%), povidone iodine (PVPI; 1%), and propamidine isethionate (PD; 0.1%) to suppress trophozoite formation of Acantamoeba castellanii and Acanthamoeba hatchetti cysts on non-nutrient agar Escherichia coli plates. Of the eight off-label anti-amoebic drugs tested, only PVPI allowed for a complete suppression of trophozoite formation by drug-challenged cysts for all four Acanthamoeba isolates in all five biological replicates. Drugs such as NM, PD, and PHMB repeatedly suppressed trophozoite formation with some, but not all, tested Acanthamoeba isolates, while other drugs such as CH, DD, and MF failed to exert a relevant effect on the excystation capacities of the tested Acanthamoeba isolates in most, if not all, of our repetitions. Our findings suggest that pre-testing of the AK isolate with the non-nutrient agar E. coli plate assay against the anti-amoebic drug intended for treatment should be performed to confirm that the selected drug is cysticidal for the Acanthamoeba isolate.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectAcanthamoeba keratitiseng
dc.subjectoff-label anti-amoebic drugseng
dc.subjectnon-nutrient agar escherichia coli plate assayeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleEfficacy of Off-Label Anti-Amoebic Agents to Suppress Trophozoite Formation of Acanthamoeba spp. on Non-Nutrient Agar Escherichia Coli Platesnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/12121-2
dc.type.versionpublishedVersionnone
local.edoc.container-titleMicroorganismsnone
local.edoc.container-issn2076-2607none
local.edoc.pages10none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/journal/microorganismsnone
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume10none
local.edoc.container-issue8none
local.edoc.container-reportyear2022none
dc.description.versionPeer Reviewednone

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