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2022-05-26Zeitschriftenartikel
Epstein-Barr Virus-Encoded BILF1 Orthologues From Porcine Lymphotropic Herpesviruses Display Common Molecular Functionality
dc.contributor.authorMavri, Maša
dc.contributor.authorKubale, Valentina
dc.contributor.authorDepledge, Daniel P.
dc.contributor.authorZuo, Jianmin
dc.contributor.authorHuang, Christene A.
dc.contributor.authorBreuer, Judith
dc.contributor.authorVrecl, Milka
dc.contributor.authorJarvis, Michael A.
dc.contributor.authorJovičić, Eva Jarc
dc.contributor.authorPetan, Toni
dc.contributor.authorEhlers, Bernhard
dc.contributor.authorRosenkilde, Mette M.
dc.contributor.authorSpiess, Katja
dc.date.accessioned2024-09-10T11:39:09Z
dc.date.available2024-09-10T11:39:09Z
dc.date.issued2022-05-26none
dc.identifier.other10.3389/fendo.2022.862940
dc.identifier.urihttp://edoc.rki.de/176904/12131
dc.description.abstractInfection of immunosuppressed transplant patients with the human γ-herpesvirus Epstein-Barr virus (EBV) is associated with post-transplant lymphoproliferative disease (PTLD), an often fatal complication. Immunosuppressed miniature pigs infected with γ-herpesvirus porcine lymphotropic herpesvirus 1 (PLHV1) develop a similar disease, identifying pigs as a potential preclinical model for PTLD in humans. BILF1 is a G protein-coupled receptor (GPCR) encoded by EBV with constitutive activity linked to tumorigenesis and immunoevasive function downregulating MHC-I. In the present study, we compared BILF1-orthologues encoded by the three known PLHVs (PLHV1-3) with EBV-BILF1 to determine pharmacological suitability of BILF1 orthologues as model system to study EBV-BILF1 druggability. Cell surface localization, constitutive internalization, and MHC-I downregulation as well as membrane proximal constitutive Gαi signaling patterns were conserved across all BILFs. Only subtle differences between the individual BILFs were observed in downstream transcription factor activation. Using Illumina sequencing, PLHV1 was observed in lymphatic tissue from PTLD-diseased, but not non-diseased pigs. Importantly, these tissues showed enhanced expression of PLHV1-BILF1 supporting its involvement in PTLD infection.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectEppstein-Barr viruseng
dc.subjectporcine lymphotropic herpesviruses (PLHV)eng
dc.subjectBILF1eng
dc.subjectG protein signalingeng
dc.subjectMHC class 1eng
dc.subjectdrug targeteng
dc.subjectpost-transplat lymphoproliferative diseaseeng
dc.subjectin-vivo modeleng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleEpstein-Barr Virus-Encoded BILF1 Orthologues From Porcine Lymphotropic Herpesviruses Display Common Molecular Functionalitynone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/12131-7
dc.type.versionpublishedVersionnone
local.edoc.container-titleFrontiers in Endocrinologynone
local.edoc.container-issn1664-2392none
local.edoc.pages16none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.frontiersin.org/journals/endocrinologynone
local.edoc.container-publisher-nameFrontiers Meadia S.A.none
local.edoc.container-volume13none
local.edoc.container-reportyear2022none
dc.description.versionPeer Reviewednone

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