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2022-12-26Zeitschriftenartikel
Extensive testing of a multi-locus sequence typing scheme for Giardia duodenalis assemblage A confirms its good discriminatory power
dc.contributor.authorKlotz, Christian
dc.contributor.authorSannella, Anna Rosa
dc.contributor.authorWeisz, Filip
dc.contributor.authorChaudhry, Umer
dc.contributor.authorSroka, Jacek
dc.contributor.authorTůmová, Pavla
dc.contributor.authorNohýnková, Eva
dc.contributor.authorIgnatius, Ralf
dc.contributor.authorAebischer, Toni
dc.contributor.authorBetson, Martha
dc.contributor.authorTroell, Karin
dc.contributor.authorCacciò, Simone
dc.date.accessioned2024-09-13T14:06:15Z
dc.date.available2024-09-13T14:06:15Z
dc.date.issued2022-12-26none
dc.identifier.other10.1186/s13071-022-05615-x
dc.identifier.urihttp://edoc.rki.de/176904/12168
dc.description.abstractBackground The flagellated parasite Giardia duodenalis is a major and global cause of diarrhoeal disease. Eight genetically very distinct groups, known as assemblages A to H, have been recognized in the G. duodenalis species complex, two of which (assemblages A and B) infect humans and other mammalian hosts. Informative typing schemes are essential to understand transmission pathways, characterize outbreaks and trace zoonotic transmission. In this study, we evaluated a published multi-locus sequence typing (MLST) scheme for G. duodenalis assemblage A, which is based on six polymorphic markers. Methods We genotyped 60 human-derived and 11 animal-derived G. duodenalis isolates collected in Europe and on other continents based on the published protocol. After retrieving previously published genotyping data and excluding isolates whose sequences showed allelic sequence heterozygosity, we analysed a dataset comprising 146 isolates. Results We identified novel variants at five of the six markers and identified 78 distinct MLST types in the overall dataset. Phylogenetic interpretation of typing data confirmed that sub-assemblage AII only comprises human-derived isolates, whereas sub-assemblage AI comprises all animal-derived isolates and a few human-derived isolates, suggesting limited zoonotic transmission. Within sub-assemblage AII, isolates from two outbreaks, which occurred in Sweden and Italy, respectively, had unique and distinct MLST types. Population genetic analysis showed a lack of clustering by geographical origin of the isolates. Conclusion The MLST scheme evaluated provides sufficient discriminatory power for epidemiological studies of G. duodenalis assemblage Aeng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectmolecular epidemiologyeng
dc.subjectzoonotic transmissioneng
dc.subjectsource tracingeng
dc.subjectMLSTeng
dc.subjectOutbreakeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleExtensive testing of a multi-locus sequence typing scheme for Giardia duodenalis assemblage A confirms its good discriminatory powernone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/12168-3
dc.type.versionpublishedVersionnone
local.edoc.container-titleParasites & Vectorsnone
local.edoc.container-issn1756-3305none
local.edoc.pages7none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://parasitesandvectors.biomedcentral.com/none
local.edoc.container-publisher-nameSpringer Naturenone
local.edoc.container-volume15none
local.edoc.container-reportyear2022none
dc.description.versionPeer Reviewednone

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