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2022-05-03Zeitschriftenartikel
Genomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirus
dc.contributor.authorDiaz-Cánova, Diana
dc.contributor.authorMoens, Ugo L.
dc.contributor.authorBrinkmann, Annika
dc.contributor.authorNitsche, Andreas
dc.contributor.authorOkeke, Malachy Ifeanyi
dc.date.accessioned2024-09-19T08:24:45Z
dc.date.available2024-09-19T08:24:45Z
dc.date.issued2022-05-03none
dc.identifier.other10.3389/fmicb.2022.868887
dc.identifier.urihttp://edoc.rki.de/176904/12225
dc.description.abstractOrthopoxviruses (OPXVs) not only infect their natural hosts, but some OPXVs can also cause disease in humans. Previously, we partially characterized an OPXV isolated from an 18-year-old male living in Northern Norway. Restriction enzyme analysis and partial genome sequencing characterized this virus as an atypical cowpox virus (CPXV), which we named CPXV-No-H2. In this study, we determined the complete genome sequence of CPXV-No-H2 using Illumina and Nanopore sequencing. Our results showed that the whole CPXV-No-H2 genome is 220,276 base pairs (bp) in length, with inverted terminal repeat regions of approximately 7 kbp, containing 217 predicted genes. Seventeen predicted CPXV-No-H2 proteins were most similar to OPXV proteins from the Old World, including Ectromelia virus (ECTV) and Vaccinia virus, and North America, Alaskapox virus (AKPV). CPXV-No-H2 has a mosaic genome with genes most similar to other OPXV genes, and seven potential recombination events were identified. The phylogenetic analysis showed that CPXV-No-H2 formed a separate clade with the German CPXV isolates CPXV_GerMygEK938_17 and CPXV_Ger2010_MKY, sharing 96.4 and 96.3% nucleotide identity, respectively, and this clade clustered closely with the ECTV-OPXV Abatino clade. CPXV-No-H2 is a mosaic virus that may have arisen out of several recombination events between OPXVs, and its phylogenetic clustering suggests that ECTV-Abatino-like cowpox viruses form a distinct, new clade of cowpox viruses.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectpoxviruseng
dc.subjectphylogeneticseng
dc.subjectfennoscandianeng
dc.subjectNorwayeng
dc.subjectrecombinationeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleGenomic Sequencing and Analysis of a Novel Human Cowpox Virus With Mosaic Sequences From North America and Old World Orthopoxvirusnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/12225-6
dc.type.versionpublishedVersionnone
local.edoc.container-titleFrontiers in Microbiologynone
local.edoc.container-issn1664-302Xnone
local.edoc.pages14none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.frontiersin.org/journals/microbiologynone
local.edoc.container-publisher-nameFrontiers Meadia S.A.none
local.edoc.container-volume13none
local.edoc.container-reportyear2022none
dc.description.versionPeer Reviewednone

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