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2022-03-09Zeitschriftenartikel
RespiCoV: Simultaneous identification of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and 46 respiratory tract viruses and bacteria by amplicon-based Oxford-Nanopore MinION sequencing
dc.contributor.authorBrinkmann, Annika
dc.contributor.authorUddin, Steven
dc.contributor.authorUlm, Sophie-Luisa
dc.contributor.authorPape, Katharina
dc.contributor.authorFörster, Sophie
dc.contributor.authorEnan, Khalid
dc.contributor.authorNourlil, Jalal
dc.contributor.authorKrause, Eva
dc.contributor.authorSchaade, Lars
dc.contributor.authorMichel, Janine
dc.contributor.authorNitsche, Andreas
dc.date.accessioned2024-09-19T15:52:59Z
dc.date.available2024-09-19T15:52:59Z
dc.date.issued2022-03-09none
dc.identifier.other10.1371/journal.pone.0264855
dc.identifier.urihttp://edoc.rki.de/176904/12247
dc.description.abstractSince December 2019 the world has been facing the outbreak of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Identification of infected patients and discrimination from other respiratory infections have so far been accomplished by using highly specific real-time PCRs. Here we present a rapid multiplex approach (RespiCoV), combining highly multiplexed PCRs and MinION sequencing suitable for the simultaneous screening for 41 viral and five bacterial agents related to respiratory tract infections, including the human coronaviruses NL63, HKU1, OC43, 229E, Middle East respiratory syndrome coronavirus, SARS-CoV, and SARS-CoV-2. RespiCoV was applied to 150 patient samples with suspected SARS-CoV-2 infection and compared with specific real-time PCR. Additionally, several respiratory tract pathogens were identified in samples tested positive or negative for SARS-CoV-2. Finally, RespiCoV was experimentally compared to the commercial RespiFinder 2SMART multiplex screening assay (PathoFinder, The Netherlands).eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleRespiCoV: Simultaneous identification of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and 46 respiratory tract viruses and bacteria by amplicon-based Oxford-Nanopore MinION sequencingnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/12247-4
dc.type.versionpublishedVersionnone
local.edoc.container-titlePLOS ONEnone
local.edoc.container-issn1932-6203none
local.edoc.pages11none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://journals.plos.org/plosone/none
local.edoc.container-publisher-namePLOSnone
local.edoc.container-volume17none
local.edoc.container-issue3none
local.edoc.container-reportyear2022none
dc.description.versionPeer Reviewednone

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