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2012-05-03Zeitschriftenartikel DOI: 10.1371/journal.pone.0036291
First International External Quality Assessment Study on Molecular and Serological Methods for Yellow Fever Diagnosis
dc.contributor.authorDomingo, Cristina
dc.contributor.authorEscadafal, Camille
dc.contributor.authorRumer, Leonid
dc.contributor.authorMéndez, Jairo A.
dc.contributor.authorGarcía, Paquita
dc.contributor.authorSall, Amadou A.
dc.contributor.authorTeichmann, Anette
dc.contributor.authorMantke, Oliver Donoso
dc.contributor.authorNiedrig, Matthias
dc.date.accessioned2018-05-07T15:41:26Z
dc.date.available2018-05-07T15:41:26Z
dc.date.created2012-06-06
dc.date.issued2012-05-03none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reodm88Ofstvo/PDF/2857cvhtmt82.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1230
dc.description.abstractObjective: We describe an external quality assurance (EQA) study designed to assess the efficiency and accurateness of molecular and serological methods used by expert laboratories performing YF diagnosis. Study Design: For molecular diagnosis evaluation, a panel was prepared of 14 human plasma samples containing specific RNA of different YFV strains (YFV-17D, YFV South American strain [Brazil], YFV IvoryC1999 strain), and specificity samples containing other flaviviruses and negative controls. For the serological panel, 13 human plasma samples with anti-YFVspecific antibodies against different strains of YFV (YFV-17D strain, YFV IvoryC1999 strain, and YFV Brazilian strain), as well as specificity and negative controls, were included. Results: Thirty-six laboratories from Europe, the Americas, Middle East, and Africa participated in these EQA activities. Only 16% of the analyses reported met all evaluation criteria with optimal performance. Serial dilutions of YFV-17D showed that in general the methodologies reported provided a suitable sensitivity. Failures were mainly due to the inability to detect wild-type strains or the presence of false positives. Performance in the serological diagnosis varied, mainly depending on the methodology used. Anti-YFV IgM detection was not performed in 16% of the reports using IIF or ELISA techniques, although it is preferable for the diagnosis of YFV acute infections. A good sensitivity profile was achieved in general; however, in the detection of IgM antibodies a lack of sensitivity of anti-YFV antibodies against the vaccine strain 17D was observed, and of the anti-YFV IgG antibodies against a West African strain. Neutralization assays showed a very good performance; however, the unexpected presence of false positives underlined the need of improving the running protocols. Conclusion: This EQA provides information on each laboratory’s efficacy of RT-PCR and serological YFV diagnosis techniques. The results indicate the need for improving serological and molecular diagnosis techniques and provide a follow-up of the diagnostic profiles.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectAnimalseng
dc.subjectHumanseng
dc.subjectCercopithecus aethiopseng
dc.subjectSensitivity and Specificityeng
dc.subjectSerologic Tests/standardseng
dc.subjectViral Loadeng
dc.subjectYellow Fever/immunologyeng
dc.subjectMolecular Diagnostic Techniques/methodseng
dc.subjectReverse Transcriptase Polymerase Chain Reaction/standardseng
dc.subjectSerologic Tests/methodseng
dc.subjectYellow Fever/diagnosiseng
dc.subjectYellow fever virus/geneticseng
dc.subjectVero Cellseng
dc.subjectLaboratory Proficiency Testingeng
dc.subjectMolecular Diagnostic Techniques/standardseng
dc.subjectQuality Assurance Health Careeng
dc.subjectYellow Fever/virologyeng
dc.subjectYellow fever virus/immunologyeng
dc.subject.ddc610 Medizin
dc.titleFirst International External Quality Assessment Study on Molecular and Serological Methods for Yellow Fever Diagnosis
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10024510
dc.identifier.doi10.1371/journal.pone.0036291
dc.identifier.doihttp://dx.doi.org/10.25646/1155
local.edoc.container-titlePLoS ONE
local.edoc.container-textDomingo C, Escadafal C, Rumer L, Méndez JA, García P, et al. (2012)First International External Quality Assessment Study on Molecular and Serological Methods for Yellow Fever Diagnosis.PLoS ONE 7(5): e36291.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0036291
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume7
local.edoc.container-issue5
local.edoc.container-year2012

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