Zur Kurzanzeige

2012-06-04Zeitschriftenartikel DOI: 10.1371/journal.pone.0038140
mPSQed: A Software for the Design of Multiplex Pyrosequencing Assays.
dc.contributor.authorDabrowski, Piotr Wojtek
dc.contributor.authorNitsche, Andreas
dc.date.accessioned2018-05-07T15:44:44Z
dc.date.available2018-05-07T15:44:44Z
dc.date.created2012-06-28
dc.date.issued2012-06-04none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rezPsrVUUaiTA/PDF/225dLZkl6FgGw.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1248
dc.description.abstractMolecular-based diagnostic assays are the gold standard for infectious diseases today, since they allow a rapid and sensitive identification and typing of various pathogens. While PCR can be designed to be specific for a certain pathogen, a subsequent sequence analysis is frequently required for confirmation or typing. The design of appropriate PCR-based assays is a complex task, especially when conserved discriminating polymorphisms are rare or if the number of types which need to be differentiated is high. One extremely useful but underused method for this purpose is the multiplex pyrosequencing technique. Unfortunately there is no software available to aid researchers in designing multiplex pyrosequencing assays. Here, we present mPSQed (Multiplex PyroSeQuencing EDitor), a program targeted at closing this gap. We also present the design of an exemplarily theoretical assay for the differentiation of human adenovirus types A–F using two pyrosequencing primers on two distinct PCR products, designed quickly and easily using our software.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subject.ddc610 Medizin
dc.titlemPSQed: A Software for the Design of Multiplex Pyrosequencing Assays.
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10025798
dc.identifier.doi10.1371/journal.pone.0038140
dc.identifier.doihttp://dx.doi.org/10.25646/1173
local.edoc.container-titlePLoS ONE
local.edoc.container-textDabrowski PW, Nitsche A (2012) mPSQed: A Software for the Design of Multiplex Pyrosequencing Assays. PLoS ONE 7(6): e38140.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0038140
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume7
local.edoc.container-issue6
local.edoc.container-year2012

Zur Kurzanzeige