Show simple item record

2012-06-29Zeitschriftenartikel DOI: 10.1371/journal.pone.0039928
Rapid and high-throughput detection of highly pathogenic bacteria by Ibis PLEX-ID technology
dc.contributor.authorJacob, Daniela
dc.contributor.authorSauer, Ursula
dc.contributor.authorHousley, Roberta
dc.contributor.authorWashington, Cicely
dc.contributor.authorSannes-Lowery, Kristin
dc.contributor.authorEcker, David J.
dc.contributor.authorSampath, Rangarajan
dc.contributor.authorGrunow, Roland
dc.date.accessioned2018-05-07T15:47:28Z
dc.date.available2018-05-07T15:47:28Z
dc.date.created2012-08-28
dc.date.issued2012-06-29none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reHrHCshgUis/PDF/26TfSjvKo1CxE.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1263
dc.description.abstractIn this manuscript, we describe the identification of highly pathogenic bacteria using an assay coupling biothreat group-specific PCR with electrospray ionization mass spectrometry (PCR/ESI-MS) run on an Ibis PLEX-ID high-throughput platform. The biothreat cluster assay identifies most of the potential bioterrorism-relevant microorganisms including Bacillus anthracis, Francisella tularensis, Yersinia pestis, Burkholderia mallei and pseudomallei, Brucella species, and Coxiella burnetii. DNA from 45 different reference materials with different formulations and different concentrations were chosen and sent to a service screening laboratory that uses the PCR/ESI-MS platform to provide a microbial identification service. The standard reference materials were produced out of a repository built up in the framework of the EU funded project “Establishment of Quality Assurances for Detection of Highly Pathogenic Bacteria of Potential Bioterrorism Risk” (EQADeBa). All samples were correctly identified at least to the genus level.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subject.ddc610 Medizin
dc.titleRapid and high-throughput detection of highly pathogenic bacteria by Ibis PLEX-ID technology
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10026687
dc.identifier.doi10.1371/journal.pone.0039928
dc.identifier.doihttp://dx.doi.org/10.25646/1188
local.edoc.container-titlePLoS ONE
local.edoc.container-textJacob D, Sauer U, Housley R, Washington C, Sannes-Lowery K, et al. (2012) Rapid and High-Throughput Detection of Highly Pathogenic Bacteria by Ibis PLEX-ID Technology. PLoS ONE 7(6): e39928.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0039928
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume7
local.edoc.container-issue6
local.edoc.container-year2012

Show simple item record