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2012-07-27Zeitschriftenartikel DOI: 10.1371/journal.pone.0041637
Zidovudine Exposure in HIV-1 Infected Tanzanian Women Increases Mitochondrial DNA Levels in Placenta and Umbilical Cords
dc.contributor.authorKunz, Andrea
dc.contributor.authorWurmb-Schwark, Nicole von
dc.contributor.authorSewangi, Julius
dc.contributor.authorZiske, Judith
dc.contributor.authorLau, Inga
dc.contributor.authorMbezi, Paulina
dc.contributor.authorTheuring, Stefanie
dc.contributor.authorHauser, Andrea
dc.contributor.authorDugange, Festo
dc.contributor.authorKaterna, Angela
dc.contributor.authorHarms, Gundel
dc.date.accessioned2018-05-07T15:48:24Z
dc.date.available2018-05-07T15:48:24Z
dc.date.created2012-09-03
dc.date.issued2012-07-27none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/re4YGdxLc45Q/PDF/24YuRPaX0byuo.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1268
dc.description.abstractBackground: Zidovudine (AZT) constitutes part of the recommended regimens for prevention and treatment of HIV-1 infection. At the same time, AZT as well as HIV-1 infection itself may induce mitochondrial damage. In this study, we analyzed the impact of prenatal AZT-exposure on mitochondrial alterations in HIV-infected women and their infants. Methods: Mitochondrial DNA (mtDNA) levels in placentas of HIV-1 infected Tanzanian women with and without prenatal AZT exposure, and in the umbilical cords of their AZT-exposed/unexposed infants were quantified using real-time PCR. Furthermore, we checked for the most common mitochondrial deletion in humans, the 4977 base pair deletion (dmtDNA4977) as a marker for mitochondrial stress. Results: 83 women fulfilled the inclusion criteria. 30 women had been treated with AZT (median duration 56 days; IQR 43–70 days) while 53 women had not taken AZT during pregnancy. Baseline maternal characteristics in the two groups were similar. The median mtDNA levels in placentas and umbilical cords of women (311 copies/cell) and infants (190 copies/cell) exposed to AZT were significantly higher than in AZT-unexposed women (187 copies/cell; p = 0.021) and infants (127 copies/cell; p = 0.037). The dmtDNA4977 was found in placentas of one woman of each group and in 3 umbilical cords of AZT-unexposed infants but not in umbilical cords of AZT-exposed infants. Conclusions: Antenatal AZT intake did not increase the risk for the common mitochondrial deletion dmtDNA4977. Our data suggests that AZT exposure elevates mtDNA levels in placentas and umbilical cords possibly by positively influencing the course of maternal HIV-1 infection.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectAdulteng
dc.subjectHumanseng
dc.subjectFemaleeng
dc.subjectPregnancyeng
dc.subjectMaleeng
dc.subjectInfanteng
dc.subjectYoung Adulteng
dc.subjectHIV Infections/drug therapyeng
dc.subjectHIV-1/drug effectseng
dc.subjectHIV-1/physiologyeng
dc.subjectAnti-HIV Agents/therapeutic useeng
dc.subjectHIV Infections/geneticseng
dc.subjectAnti-HIV Agents/pharmacologyeng
dc.subjectDNA Mitochondrial/metabolismeng
dc.subjectHIV Infections/embryologyeng
dc.subjectHIV Infections/metabolismeng
dc.subjectPlacenta/drug effectseng
dc.subjectPlacenta/metabolismeng
dc.subjectTanzaniaeng
dc.subjectUmbilical Cord/drug effectseng
dc.subjectUmbilical Cord/metabolismeng
dc.subjectZidovudine/pharmacologyeng
dc.subjectZidovudine/therapeutic useeng
dc.subject.ddc610 Medizin
dc.titleZidovudine Exposure in HIV-1 Infected Tanzanian Women Increases Mitochondrial DNA Levels in Placenta and Umbilical Cords
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10026746
dc.identifier.doi10.1371/journal.pone.0041637
dc.identifier.doihttp://dx.doi.org/10.25646/1193
local.edoc.container-titlePLoS ONE
local.edoc.container-textKunz A, von Wurmb-Schwark N, Sewangi J, Ziske J, Lau I, et al. (2012) Zidovudine Exposure in HIV-1 Infected Tanzanian Women Increases Mitochondrial DNA Levels in Placenta and Umbilical Cords. PLoS ONE 7(7): e41637.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0041637
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume7
local.edoc.container-issue7
local.edoc.container-year2012

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