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2007-12-01Zeitschriftenartikel DOI: 10.1099/vir.0.83187-0
Prion propagation in a nerve conduit model containing segments devoid of axons
dc.contributor.authorKratzel, Christine
dc.contributor.authorKrüger, Dominique
dc.contributor.authorBeekes, Michael
dc.date.accessioned2018-05-07T15:55:45Z
dc.date.available2018-05-07T15:55:45Z
dc.date.created2012-09-18
dc.date.issued2007-12-01none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/re48HLaaygSyU/PDF/23hTYFF1cwkp.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1308
dc.description.abstractPrions, the putative causative agents of transmissible spongiform encephalopathies, are neurotropic pathogens that spread to the central nervous system via synaptically linked neural conduits upon peripheral infection. Axons and their transport processes have been suggested as mediators of nerve-associated prion dissemination. However, the exact cellular components and molecular mechanisms underlying neural spread are unknown. This study used an established hamster scrapie model to pursue a novel experimental approach using nerve conduits containing segments devoid of neurites generated by incomplete nerve regeneration following Wallerian degeneration to probe the necessity of axons for the neural propagation of prions. For this purpose, animals were subjected to unilateral sciatic neurectomy 4 weeks before footpad inoculation with scrapie agent. The results showed that the regional nerve is the prime conduit for cerebral neuroinvasion and revealed, as evidenced by the accumulation of pathological prion protein PrP TSE, that prions can proceed along segments of peripheral neural projections without detectable axonal structures.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectAnimalseng
dc.subjectFemaleeng
dc.subjectMaleeng
dc.subjectCricetinaeeng
dc.subjectTissue Distributioneng
dc.subjectMesocricetuseng
dc.subjectBlotting Westerneng
dc.subjectPrions/isolation & purificationeng
dc.subjectPrions/metabolismeng
dc.subjectImmunohistochemistryeng
dc.subjectAxons/metabolismeng
dc.subjectBiological Transporteng
dc.subjectNeurons/metabolismeng
dc.subjectPrion Diseases/metabolismeng
dc.subject.ddc610 Medizin
dc.titlePrion propagation in a nerve conduit model containing segments devoid of axons
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10027237
dc.identifier.doi10.1099/vir.0.83187-0
dc.identifier.doihttp://dx.doi.org/10.25646/1233
local.edoc.container-titleJournal of General Virology
local.edoc.container-textChristine Kratzel, Dominique Krüger and Michael Beekes. Prion propagation in a nerve conduit model containing segments devoid of axons (2007) Journal of General Virology, 88 (12), pp. 3479-3485.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://vir.sgmjournals.org/content/88/12/3479
local.edoc.container-publisher-nameSociety for General Microbiology
local.edoc.container-volume88
local.edoc.container-issue12
local.edoc.container-year2007

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