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2024-01-20Zeitschriftenartikel
Genome sequencing of the mpox virus 2022 outbreak with amplicon-based Oxford Nanopore MinION sequencing
dc.contributor.authorBrinkmann, Annika
dc.contributor.authorPape, Katharina
dc.contributor.authorUddin, Steven
dc.contributor.authorWoelk, Niklas
dc.contributor.authorFörster, Sophie
dc.contributor.authorJessen, Heiko
dc.contributor.authorMichel, Janine
dc.contributor.authorKohl, Claudia
dc.contributor.authorSchaade, Lars
dc.contributor.authorNitsche, Andreas
dc.date.accessioned2026-02-10T09:10:01Z
dc.date.available2026-02-10T09:10:01Z
dc.date.issued2024-01-20none
dc.identifier.other10.1016/j.jviromet.2024.114888
dc.identifier.urihttp://edoc.rki.de/176904/13290
dc.description.abstractWe present an amplicon-based assay for MinION Nanopore sequencing of mpox virus (MPXV) genomes from clinical specimens, obtaining high-quality results with an average genome coverage of 99% for Ct values of up to 25, and a genome coverage of 97.1% for Ct values from 25 to 30 which are challenging to sequence. This assay is easy to implement in PCR-based workflows and provides accurate genomic data within a short time.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectOrthopox viruseseng
dc.subjectMpox viruseng
dc.subjectMinIONeng
dc.subjectNanopore sequencingeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleGenome sequencing of the mpox virus 2022 outbreak with amplicon-based Oxford Nanopore MinION sequencingnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/13290-7
dc.type.versionpublishedVersionnone
local.edoc.container-titleJournal of Virological Methodsnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameElsevier B.V.none
local.edoc.container-reportyear2024none
local.edoc.container-firstpage1none
local.edoc.container-lastpage4none
dc.description.versionPeer Reviewednone

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