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2024-03-27Zeitschriftenartikel
Gene knock-out in Mycobacterium abscessus using Streptococcus thermophilus CRISPR/Cas
dc.contributor.authorAkter, Suriya
dc.contributor.authorKamal, Elisabeth
dc.contributor.authorSchwarz, Carsten
dc.contributor.authorLewin, Astrid
dc.date.accessioned2026-02-10T09:23:18Z
dc.date.available2026-02-10T09:23:18Z
dc.date.issued2024-03-27none
dc.identifier.other10.1016/j.mimet.2024.106924
dc.identifier.urihttp://edoc.rki.de/176904/13292
dc.description.abstractThe CRISPRi system using dCas9Sth1 from Streptococcus thermophilus developed for Mycobacterium tuberculosis and M. smegmatis was modified to allow gene knock-out in M. abscessus. Efficacy of the knock-out system was evaluated by applying deletions and insertions to the mps1 gene. A comparative genomic analysis of mutants and wild type validated the target specificity.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectCRISPR/CASeng
dc.subjectGene knock-outeng
dc.subjectmps1eng
dc.subjectMutagenesiseng
dc.subjectMycobacterium abscessuseng
dc.subjectNontuberculous mycobacteriaeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleGene knock-out in Mycobacterium abscessus using Streptococcus thermophilus CRISPR/Casnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/13292-7
dc.type.versionpublishedVersionnone
local.edoc.container-titleJournal of Microbiological Methodsnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameElsevier B.V.none
local.edoc.container-reportyear2024none
local.edoc.container-firstpage1none
local.edoc.container-lastpage3none
dc.description.versionPeer Reviewednone

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