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2012-12-06Zeitschriftenartikel DOI: 10.25646/1313
Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections
dc.contributor.authorCorman, V. M.
dc.contributor.authorMüller, Marcel A.
dc.contributor.authorCostabel, U.
dc.contributor.authorTimm, J.
dc.contributor.authorBinger, T.
dc.contributor.authorMeyer, B.
dc.contributor.authorKreher, Petra
dc.contributor.authorLattwein, Erik
dc.contributor.authorEschbach-Bludau, M.
dc.contributor.authorNitsche, Andreas
dc.contributor.authorBleicker, T.
dc.contributor.authorLandt, Olfert
dc.contributor.authorSchweiger, Brunhilde
dc.contributor.authorDrexler, Jan Felix
dc.contributor.authorOsterhaus, A. D.
dc.contributor.authorHaagmans, B. L.
dc.contributor.authorDittmer, U.
dc.contributor.authorBonin, F.
dc.contributor.authorWolff, Thorsten
dc.contributor.authorDrosten, Christian
dc.date.accessioned2018-05-07T16:10:36Z
dc.date.available2018-05-07T16:10:36Z
dc.date.created2013-01-08
dc.date.issued2012-12-06none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reYmSKfV5WPo/PDF/233Ixwd0EStyQ.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1388
dc.description.abstractWe present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subject.ddc610 Medizin
dc.titleAssays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10029036
dc.identifier.doihttp://dx.doi.org/10.25646/1313
local.edoc.container-titleEuroSurveillance
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=20334
local.edoc.container-publisher-nameECDC
local.edoc.container-volume17
local.edoc.container-issue49
local.edoc.container-year2012

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