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2013-03-07Zeitschriftenartikel DOI: 10.1371/journal.pone.0058155
Subpopulations of Staphylococcus aureus Clonal Complex 121 Are Associated with Distinct Clinical Entities
dc.contributor.authorKurt, Kevin
dc.contributor.authorRasigade, Jean-Philippe
dc.contributor.authorLaurent, Frederic
dc.contributor.authorGoering, Richard V.
dc.contributor.authorZemličková, Helena
dc.contributor.authorMachova, Ivana
dc.contributor.authorStruelens, Marc J.
dc.contributor.authorZautner, Andreas E.
dc.contributor.authorHoltfreter, Silva
dc.contributor.authorBröker, Barbara
dc.contributor.authorRitchie, Stephen
dc.contributor.authorReaksmey, Sin
dc.contributor.authorLimmathurotsakul, Direk
dc.contributor.authorPeacock, Sharon J.
dc.contributor.authorCuny, Christiane
dc.contributor.authorLayer, Franziska
dc.contributor.authorWitte, Wolfgang
dc.contributor.authorNübel, Ulrich
dc.date.accessioned2018-05-07T16:17:49Z
dc.date.available2018-05-07T16:17:49Z
dc.date.created2013-03-26
dc.date.issued2013-03-07none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reZYmNLnGJbTE/PDF/24zBbP68ZQrcg.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1427
dc.description.abstractWe investigated the population structure of Staphylococcus aureus clonal complex CC121 by mutation discovery at 115 genetic housekeeping loci from each of 154 isolates, sampled on five continents between 1953 and 2009. In addition, we pyro-sequenced the genomes from ten representative isolates. The genome-wide SNPs that were ascertained revealed the evolutionary history of CC121, indicating at least six major clades (A to F) within the clonal complex and dating its most recent common ancestor to the pre-antibiotic era. The toxin gene complement of CC121 isolates was correlated with their SNP-based phylogeny. Moreover, we found a highly significant association of clinical phenotypes with phylogenetic affiliations, which is unusual for S. aureus. All isolates evidently sampled from superficial infections (including staphylococcal scalded skin syndrome, bullous impetigo, exfoliative dermatitis, conjunctivitis) clustered in clade F, which included the European epidemic fusidic-acid resistant impetigo clone (EEFIC). In comparison, isolates from deep-seated infections (abscess, furuncle, pyomyositis, necrotizing pneumonia) were disseminated in several clades, but not in clade F. Our results demonstrate that phylogenetic lineages with distinct clinical properties exist within an S. aureus clonal complex, and that SNPs serve as powerful discriminatory markers, able to identify these lineages. All CC121 genomes harboured a 41-kilobase prophage that was dissimilar to S. aureus phages sequenced previously. Community-associated MRSA and MSSA from Cambodia were extremely closely related, suggesting this MRSA arose in the region.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectHumanseng
dc.subjectPhenotypeeng
dc.subjectPhylogenyeng
dc.subjectBacterial Toxins/geneticseng
dc.subjectGenes Essentialeng
dc.subjectGenome Bacterialeng
dc.subjectPolymorphism Single Nucleotideeng
dc.subjectStaphylococcal Infections/microbiologyeng
dc.subjectStaphylococcus aureus/classificationeng
dc.subjectStaphylococcus aureus/geneticseng
dc.subject.ddc610 Medizin
dc.titleSubpopulations of Staphylococcus aureus Clonal Complex 121 Are Associated with Distinct Clinical Entities
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10029710
dc.identifier.doi10.1371/journal.pone.0058155
dc.identifier.doihttp://dx.doi.org/10.25646/1352
local.edoc.container-titlePLoS ONE
local.edoc.container-textKurt K, Rasigade J-P, Laurent F, Goering RV, Žemličková H, et al. (2013) Subpopulations of Staphylococcus aureus Clonal Complex 121 Are Associated with Distinct Clinical Entities. PLoS ONE 8(3): e58155.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0058155
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume8
local.edoc.container-issue3
local.edoc.container-year2013

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