Zur Kurzanzeige

2013-01-08Zeitschriftenartikel DOI: 10.1101/gr.147710.112
A genomic portrait of the emergence, evolution, and global spread of a methicillin-resistant Staphylococcus aureus pandemic
dc.contributor.authorHolden, Matthew T.G.
dc.contributor.authorHsu, Li-Yang
dc.contributor.authorKurt, Kevin
dc.contributor.authorWeinert, Lucy A.
dc.contributor.authorMather, Alison E.
dc.contributor.authorHarris, Simon R.
dc.contributor.authorStrommenger, Birgit
dc.contributor.authorLayer, Franziska
dc.contributor.authorWitte, Wolfgang
dc.contributor.authorLencastre, Herminia de
dc.contributor.authorSkov, Robert
dc.contributor.authorWesth, Henrik
dc.contributor.authorZemličková, Helena
dc.contributor.authorCoombs, Geoffrey
dc.contributor.authorKearns, Angela M.
dc.contributor.authorHill, Robert L.R.
dc.contributor.authorEdgeworth, Jonathan
dc.contributor.authorGould, Ian
dc.contributor.authorGant, Vanya
dc.contributor.authorCooke, Jonathan
dc.contributor.authorEdwards, Giles F.
dc.contributor.authorMcAdam, Paul R.
dc.contributor.authorTempleton, Kate E.
dc.contributor.authorMcCann, Angela
dc.contributor.authorNübel, Ulrich
dc.date.accessioned2018-05-07T16:18:23Z
dc.date.available2018-05-07T16:18:23Z
dc.date.created2013-03-26
dc.date.issued2013-01-08none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reJfg93Lb4kM/PDF/26uD9faLIPiX2.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1430
dc.description.abstractThe widespread use of antibiotics in association with high-density clinical care has driven the emergence of drug-resistant bacteria that are adapted to thrive in hospitalized patients. Of particular concern are globally disseminated methicillin-resistant Staphylococcus aureus (MRSA) clones that cause outbreaks and epidemics associated with health care. The most rapidly spreading and tenacious health-care-associated clone in Europe currently is EMRSA-15, which was first detected in the UK in the early 1990s and subsequently spread throughout Europe and beyond. Using phylogenomic methods to analyze the genome sequences for 193 S. aureus isolates, we were able to show that the current pandemic population of EMRSA-15 descends from a health-care-associated MRSA epidemic that spread throughout England in the 1980s, which had itself previously emerged from a primarily community-associated methicillin-sensitive population. The emergence of fluoroquinolone resistance in this EMRSA-15 subclone in the English Midlands during the mid-1980s appears to have played a key role in triggering pandemic spread, and occurred shortly after the first clinical trials of this drug. Genome-based coalescence analysis estimated that the population of this subclone over the last 20 yr has grown four times faster than its progenitor. Using comparative genomic analysis we identified the molecular genetic basis of 99.8% of the antimicrobial resistance phenotypes of the isolates, highlighting the potential of pathogen genome sequencing as a diagnostic tool. We document the genetic changes associated with adaptation to the hospital environment and with increasing drug resistance over time, and how MRSA evolution likely has been influenced by country-specific drug use regimens.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectHumanseng
dc.subjectGenotypeeng
dc.subjectPhylogenyeng
dc.subjectGenomicseng
dc.subjectCluster Analysiseng
dc.subjectGenome Bacterialeng
dc.subjectDrug Resistance Bacterial/geneticseng
dc.subjectGreat Britain/epidemiologyeng
dc.subjectMethicillin-Resistant Staphylococcus aureus/classificationeng
dc.subjectMethicillin-Resistant Staphylococcus aureus/geneticseng
dc.subjectPandemicseng
dc.subjectPhylogeographyeng
dc.subjectStaphylococcal Infections/epidemiologyeng
dc.subjectStaphylococcal Infections/transmissioneng
dc.subject.ddc610 Medizin
dc.titleA genomic portrait of the emergence, evolution, and global spread of a methicillin-resistant Staphylococcus aureus pandemic
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10029745
dc.identifier.doi10.1101/gr.147710.112
dc.identifier.doihttp://dx.doi.org/10.25646/1355
local.edoc.container-titleGenome Research
local.edoc.container-textFreely available online through the Genome Research Open Access option.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://genome.cshlp.org/content/early/2013/02/24/gr.147710.112
local.edoc.container-publisher-nameCold Spring Harbor Laboratory Press
local.edoc.container-volume23
local.edoc.container-issue4
local.edoc.container-year2013

Zur Kurzanzeige