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2013-02-20Zeitschriftenartikel DOI: 10.1186/1743-422X-10-61
Comparison of host cell gene expression in cowpox, monkeypox or vaccinia virus-infected cells reveals virus-specific regulation of immune response genes
dc.contributor.authorBourquain, Daniel
dc.contributor.authorDabrowski, Piotr Wojtek
dc.contributor.authorNitsche, Andreas
dc.date.accessioned2018-05-07T16:19:31Z
dc.date.available2018-05-07T16:19:31Z
dc.date.created2013-03-27
dc.date.issued2013-02-20none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rewYGy1ffI43w/PDF/25ws97dvxXEEA.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1436
dc.description.abstractBackground: Animal-borne orthopoxviruses, like monkeypox, vaccinia and the closely related cowpox virus, are all capable of causing zoonotic infections in humans, representing a potential threat to human health. The disease caused by each virus differs in terms of symptoms and severity, but little is yet know about the reasons for these varying phenotypes. They may be explained by the unique repertoire of immune and host cell modulating factors encoded by each virus. In this study, we analysed the specific modulation of the host cell’s gene expression profile by cowpox, monkeypox and vaccinia virus infection. We aimed to identify mechanisms that are either common to orthopoxvirus infection or specific to certain orthopoxvirus species, allowing a more detailed description of differences in virus-host cell interactions between individual orthopoxviruses. To this end, we analysed changes in host cell gene expression of HeLa cells in response to infection with cowpox, monkeypox and vaccinia virus, using whole-genome gene expression microarrays, and compared these to each other and to non-infected cells. Results: Despite a dominating non-responsiveness of cellular transcription towards orthopoxvirus infection, we could identify several clusters of infection-modulated genes. These clusters are either commonly regulated by orthopoxvirus infection or are uniquely regulated by infection with a specific orthopoxvirus, with major differences being observed in immune response genes. Most noticeable was an induction of genes involved in leukocyte migration and activation in cowpox and monkeypox virus-infected cells, which was not observed following vaccinia virus infection. Conclusion: Despite their close genetic relationship, the expression profiles induced by infection with different orthopoxviruses vary significantly. It may be speculated that these differences at the cellular level contribute to the individual characteristics of cowpox, monkeypox and vaccinia virus infections in certain host species.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectCowpox viruseng
dc.subjectOrthopoxviruseng
dc.subjectVaccinia viruseng
dc.subjectMonkeypox viruseng
dc.subjectMicroarrayeng
dc.subjectGene expressioneng
dc.subjectHost cell responseeng
dc.subject.ddc610 Medizin
dc.titleComparison of host cell gene expression in cowpox, monkeypox or vaccinia virus-infected cells reveals virus-specific regulation of immune response genes
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10029809
dc.identifier.doi10.1186/1743-422X-10-61
dc.identifier.doihttp://dx.doi.org/10.25646/1361
local.edoc.container-titleVirology Journal
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.virologyj.com/content/10/1/61
local.edoc.container-publisher-nameBioMedCentral
local.edoc.container-volume10
local.edoc.container-issue61
local.edoc.container-year2013

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