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2013-05-23Zeitschriftenartikel DOI: 10.1371/journal.pntd.0002244
International External Quality Assessment of Molecular Detection of Rift Valley Fever Virus
dc.contributor.authorEscadafal, Camille
dc.contributor.authorPaweska, Janusz T.
dc.contributor.authorGrobbelaar, Antoinette
dc.contributor.authorRoux, Chantel le
dc.contributor.authorBouloy, Michèle
dc.contributor.authorPatel, Pranav
dc.contributor.authorTeichmann, Anette
dc.contributor.authorMantke, Oliver Donoso
dc.contributor.authorNiedrig, Matthias
dc.date.accessioned2018-05-07T16:52:13Z
dc.date.available2018-05-07T16:52:13Z
dc.date.created2013-07-24
dc.date.issued2013-05-23none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rexscpz3vPzs/PDF/21vkepEPkM6M.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1615
dc.description.abstractRift Valley fever (RVF) is a viral zoonosis that primarily affects animals resulting in considerable economic losses due to death and abortions among infected livestock. RVF also affects humans with clinical symptoms ranging from an influenza-like illness to a hemorrhagic fever. Over the past years, RVF virus (RVFV) has caused severe outbreaks in livestock and humans throughout Africa and regions of the world previously regarded as free of the virus. This situation prompts the need to evaluate the diagnostic capacity and performance of laboratories worldwide. Diagnostic methods for RVFV detection include virus isolation, antigen and antibody detection methods, and nucleic acid amplification techniques. Molecular methods such as reverse-transcriptase polymerase chain reaction and other newly developed techniques allow for a rapid and accurate detection of RVFV. This study aims to assess the efficiency and accurateness of RVFV molecular diagnostic methods used by expert laboratories worldwide. Thirty expert laboratories from 16 countries received a panel of 14 samples which included RVFV preparations representing several genetic lineages, a specificity control and negative controls. In this study we present the results of the first international external quality assessment (EQA) for the molecular diagnosis of RVF. Optimal results were reported by 64% of the analyses, 21% of the analyses achieved acceptable results and 15% of the results revealed that there is need for improvement. Evenly good performances were achieved by specific protocols which can therefore be recommended as an accurate molecular protocol for the diagnosis of RVF. Other protocols showed uneven performances revealing the need for improved optimization and standardization of these protocols.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subject.ddc610 Medizin
dc.titleInternational External Quality Assessment of Molecular Detection of Rift Valley Fever Virus
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10032122
dc.identifier.doi10.1371/journal.pntd.0002244
dc.identifier.doihttp://dx.doi.org/10.25646/1540
local.edoc.container-titlePLoS Neglected Tropical Diseases
local.edoc.container-textEscadafal C, Paweska JT, Grobbelaar A, le Roux C, Bouloy M, et al. (2013) International External Quality Assessment of Molecular Detection of Rift Valley Fever Virus. PLoS Negl Trop Dis 7(5): e2244.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0002244
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume7
local.edoc.container-issue5
local.edoc.container-year2013

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